Effect of Chaibei Zhixian Decoction and Its Absorption Component Peimine on Content of Carbamazepine in Rat Brain and Investigation of Its Mechanism
10.13422/j.cnki.syfjx.20191939
- VernacularTitle: 柴贝止痫汤及其吸收成分贝母素甲对大鼠脑内卡马西平含量的影响及其机制
- Author:
Chong-chong LIU
1
;
Jiang-yan SUN
2
;
Xiao-ke DONG
1
;
Kai-yue WANG
1
;
Zhong-hao LI
1
;
Jing WU
2
;
Li-li LI
1
;
Jin-min LIU
1
Author Information
1. Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
2. Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
intractable epilepsy;
Chaibei Zhixian decoction;
peimine;
P-glycoprotein;
10,11-epoxidation of carbamazepine
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):32-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Chaibei Zhixian decoction and peimine on Carbamazepine (CBZ) concentration, P-glycoprotein (P-gp) and multi drug resistance 1(MDR1) expression in the brain tissues of rats with refractory epilepsy, and to understand the contribution of Peimine in the compound prescription to treat the refractory epilepsy. Method:Epilepsy rat models were established by injecting kainic acid (KA) in the lateral ventricle. The successfully modeled rats were randomly divided into model group, CBZ group(0.12 g·kg-1),Chaibei Zhixian decoction+CBZ group(8.39 g·kg-1+0.12 g·kg-1), peimine+CBZ group(0.01 g·kg-1+0.12 g·kg-1) and sham operation group. After 60 days of intervention, the expression levels of P-glycoprotein (P-gp) and MDR1b mRNA in the brain cortex were detected by Western blot and quantitative real\|time fluorescence polymerase chain reaction(Real-time PCR),the contents of CBZ and 10,11-epoxidation of carbamazepine (CBZE) were measured by liquid chromatography-mass spectrometry (LC-MS). Result:Compared with sham group, the expression of P-gp/MDR1 in the cortex of model group was significantly increased (P<0.05, P<0.01).Compared with model group, the P-gp/MDR1 level in CBZ group was increased.The expression of P-gp/MDR1 in the cortex of Chaibei Zhixian decoction+CBZ group and peimine+CBZ group was reduced. Compared with CBZ group, The expression of P-gp/MDR1 was significantly decreased in the cortex of Chaibei Zhixian decoction+CBZ group and peimine+CBZ group (P<0.05,P<0.01), and the content of CBZ and CBZE in the brain of peimine+CBZ group was significantly increased (P<0.05,P<0.01), and the content of CBZE in the brain of the Chaibei Zhixian decoction+CBZ group was significantly increased (P<0.05).Compared with the Chaibei Zhixian decoction+CBZ group,the content of CBZ and CBZE in the brain of the peimine+CBZ group was increased in rats, but the difference was not statistically significant. Conclusion:Chaibei Zhixian decoction and peimine may increase the content of CBZ and CBZE in the brain tissues in rats with intractable epilepsy by reducing the expression of MDR1/P-gp in the cortex.
