Effect of Serum Containing Buyang Huanwu Tang on Endothelial-to- mesenchymal Transition of Human Pulmonary Artery Endothelial Cells
10.13422/j.cnki.syfjx.20192036
- VernacularTitle: 补阳还五汤含药血清对人肺动脉内皮细胞间质转化的影响
- Author:
Jing-lian QU
1
;
Han-jie YANG
1
;
Hui-liang ZHAO
1
Author Information
1. Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
- Publication Type:Research Article
- Keywords:
Buyang Huanwu Tang;
idiopathic pulmonary fibrosis;
human pulmonary artery endothelial cells;
endothelial-to-mesenchymal transition
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(19):1-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of serum containing Buyang Huanwu Tang on endothelial-to-mesenchymal transition(EndMT) in human pulmonary artery endothelial cells (HPAEC), and make further analysis on its mechanism from the perspective of the signal transduction of Jagged1/Notch1. Method:Rabbit serum containing Buyang Huanwu Tang was prepared by gavage with dosage of 53.36 g·kg-1·d-1, and blank serum was prepared by gavage with same volume of normal saline. The HPAECs cultured in vitro, EndMT model was established by the transforming growth factor-β1(TGF-β1) induced, which were divided into five groups:the control group (10%blank serum), the model group (10%blank serum+TGF-β1), the serum containing high-dose Buyang Huanwu Tang group (10%medicated serum + TGF-β1), the medium-dose group (5%medicated serum + 5%blank serum medicated + TGF-β1) and the low-dose group(2.5%medicated serum+7.5%blank serum+ TGF-β1). Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) method. Cell migration was detected by transwell and scratch assay. The endothelial markers platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), vascular endothelial cadherin (VE-cadherin) and the mesenchymal markers fibroblast-specific protein 1 (FSP1), α-smooth muscle actin (α-SMA) were observed by immunofluorescence assay. The expression levels of Notch1, Jagged1 and CBF1 were detected by Western blot assay. Result:Compared with the control group, the proliferation and migration abilities of the HPAEC cells in model group were enhanced (P<0.01), and the expressions of CD31 and VE-cadherin were decreased, while the expressions of FSP1 and α-SMA were increased. Further study found that the expressions of Notch1, Jagged1 and CBF1 were up-regulated (P<0.01). After the intervention of the serum containing Buyang Huanwu Tang, compared with model group, the proliferation and migration abilities of cells were decreased (P<0.05,P<0.01), and the expressions of CD31 and VE-cadherin were on the rise, while the expressions of FSP1and α-SMA were on the decline. The expressions of Notch1, Jagged1 and CBF1 were also significantly lower than those in model group (P<0.05,P<0.01). With the increase of serum concentration, the effect was more obvious. Conclusion:The serum containing Buyang Huanwu Tang can partly inhibit the EndMT in human pulmonary artery endothelial cells, which may be related to the regulation effect of Jagged1/Notch1 signaling.
