Anti-inflammatory Effect of Dendrobii Huoshanense Herba
10.13422/j.cnki.syfjx.20192004
- VernacularTitle: 霍山石斛的抗炎作用
- Author:
Meng-meng WANG
1
;
Zhao-jie JI
1
;
Jiang-hua GAN
1
;
Nian-jun YU
1
;
Wei-dong CHEN
1
;
Dai-yin PENG
1
;
Lan HAN
1
Author Information
1. School of Pharmacy of Anhui University of Chinese Medicine, Anhui Province Key Laboratory of Chinese Medicinal Compounds, Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei 230012, China
- Publication Type:Research Article
- Keywords:
Dendrobii Huoshanense Herba;
anti-inflammatory effect;
ear swelling;
foot swelling;
cotton ball granuloma
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(20):76-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the anti-inflammatory effect of Dendrobii Huoshanense Herba. Method:Totally 60 Kunming mice were randomly divided into normal group, model group, high, middle and low-dose Dendrobii Huoshanense Herba groups (DHS-H, DHS-M, DHS-L, 4, 2, 1 g·kg-1) and dexamethasone acetate group (DXMS, 0.01 g·kg-1). The rats were intragastrically administered for 14 days. After the last administration for 1 h, a total of 20 μL of xylene was added to both sides of the right ear center of the mice to establish the ear swelling model. All of the mice were decapitated 1 h later, and the ear swelling inhibition rate of each group were calculated. Totally 36 healthy SD rats were divided into normal model, model group, DHS-H,DHS-M,DHS-L groups (2.8, 1.4, 0.7 g·kg-1) and DXMS acetate group. The rats were intragastrically administered for 7 days. One hour after the last intragastric administration, a foot swelling model was established through subcutaneous injection of 10%fresh egg white in the right hind limb toe of each group. The right hind paw circumference of each rat was measured at 0, 0.5, 1, 2, 3, 4, 5 h, and the paw swelling of the rats was calculated. Totally 60 SD rats were implanted subcutaneously with sterile dry cotton balls in the bilateral groin and grouped as above. All of the rats were intragastrically administered for 14 days since the next day. After the last administration, cotton balls were taken out, and the inhibition rate of granuloma was calculated. And the contents of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-γ (IFN-γ) in the serum of each group were detected. Result:Compared with the normal group, the ear swelling rate, the foot swelling degree were significantly increased in the model group(P<0.01). Compared with the model group, the swelling rate of mouse ears in high, middle and low-dose Dendrobii Huoshanense Herba groups reduced significantly (P<0.01). The swelling degrees of the high and middle-dose groups were significantly decreased (P<0.05, P<0.01). The dry weight of cotton buds in each group was significantly decreased (P<0.01), with no significant change in thymus and spleen index. Compared with the normal group, the serum levels of TNF-α, IL-1β, IL-2, IL-6, and IFN-γ in the model group were higher than those in the normal group (P<0.01). And compared with model group, the levels of TNF-α, IL-1β, IL-2, IL-6, and IFN-γ in Dendrobii Huoshanense Herba group and positive drug group were decreased significantly (P<0.05, P<0.01). Conclusion:Dendrobii Huoshanense Herba could effectively inhibit acute and chronic inflammatory reactions.
