Protective Effect of Linggui Zhugan Tang on Bone Injury in ob/ob Mice Based on Gut Microbiota
10.13422/j.cnki.syfjx.20191904
- VernacularTitle: 基于肠道菌群探讨苓桂术甘汤对瘦素缺陷代谢紊乱模型小鼠的骨保护作用
- Author:
Li-ping LIU
1
;
Ran LI
1
;
Nan JIANG
1
;
Lan-chuan ZHANG
1
;
Song GU
1
Author Information
1. Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China
- Publication Type:Research Article
- Keywords:
leptin;
metabolic disorder;
bone formation;
gut microbiota;
Linggui Zhugan Tang
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(20):19-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Linggui Zhugan Tang on gut microbiota in ob/ob mice, in order to explore whether its bone protective effect is related to its regulatory effect of gut microbiota. Method:The ob/ob mice were divided into control group, model group, Linggui Zhugan Tang group, and Atorvastatin group. After administration for 4 weeks, morphological changes in intestine and bone were observed by htoxylin eosin(HE) staining. The protein expression of alkaline phosphatase(ALP) was detected by Western blot in bone. The expression of farniol X receptor(FXR) was detected by in immunofluorescence staining. And the content of interleukin-6(IL-6) in serum was analyzed by the enzyme-linked immunosorbent assay(ELISA) method. Gut microbiota was analyzed by 16S rRNA. Result:Compared with control group, the trabecular bone of the femur became thinner, the bone marrow cavity was infiltrated with fat, and the epithelium of the small intestine was damaged in the model group. The protein expressions of ALP and FXR decreased, while the content of IL-6 in serum was increased in the model group. Compared with model group, the protein expressions of ALP and FXR increased, while the content of IL-6 in serum was decreased in the Linggui Zhugan Tang group. At the genus level, compared with control group, the abundance of Lachnospiraceae_UCG-001 increased (P<0.05), while that of norank_f__Bacteroidales_S24-7_group decreased in model group (P<0.01). Lachnospiraceae_UCG-001 was positively correlated with the weight of body (r=0.661), while norank_f__Bacteroidales_S24-7_group was negatively correlated with the weight of body (r=-0.622). Conclusion:Linggui Zhugan Tang has a protective effect on bone injury in ob/ob mice, and its mechanism may be related to its regulation of gut microbiota.
