Analysis of Mechanism of Phytolaccae Radix and Its Processed Products on Doxorubicin-induced Nephropathy in Rats
10.13422/j.cnki.syfjx.20191448
- VernacularTitle: 商陆及其不同炮制品对阿霉素肾病大鼠的作用机制分析
- Author:
Xiao-ming QI
1
;
Jun-nan MA
2
;
Xiang-long MENG
1
;
Shuo-sheng ZHANG
1
Author Information
1. Shanxi University of Chinese Medicine, Jinzhong 030619, China
2. Dongguk University, Gyeongju 780701, Korea
- Publication Type:Research Article
- Keywords:
Phytolaccae Radix;
raw products;
processed products;
esculentoside A;
nephropathy model induced by doxorubicin;
transforming growth factor-β;
urine protein
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):90-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the protective effect of Phytolaccae Radix and its processed products on nephropathy induced by doxorubicin (DOX) in rats, and explore its mechanism. Method: A rat model of nephropathy was established by a single tail intravenous injection of DOX hydrochloride. Content of esculentoside A (EsA) in Phytolaccae Radix and its processed products was determined by HPLC-ELSD. Contents of serum total protein (TP), albumin (Alb), urea nitrogen (BUN), serum creatinine (SCr), total cholesterol (TC) and urine protein (UP) were determined by enzyme-linked immunosorbent assay (ELISA). The mRNA and protein expression of transforming growth factor-β (TGF-β) in renal tissue of rats was examined by real-time quantitative polymerase chain reaction (Real-time PCR) and immunohistochemistry. Result: A single intravenous injection of DOX could induce a severe nephrotic syndrome associated with decreased serum TP, Alb and elevated serum BUN, SCr, TC, and a high urinary excretion of protein (P<0.05). Expression of BUN, SCr, TC in serum and UP in urine of model rats could be decreased by Phytolaccae Radix and its processed products to some degree, expression of TP and Alb in serum of model rats could be increased by Phytolaccae Radix and its processed products to some degree, vinegar processed products had the most significant effect. Expression of TGF-β in renal tissue of model group rats was significantly higher than that of blank group (P<0.01), which could be significantly reversed by Phytolaccae Radix processed with vinegar, Phytolaccae Radix boiled with vinegar, Phytolaccae Radix steamed with water(P<0.05, P<0.01). Conclusion: Phytolaccae Radix and its processed products can improve the symptoms of DOX nephropathy model rats in different degrees, among which the vinegar prepared products have the strongest effect, and this effect may be related to the reduction of TGF-β expression in renal tissue.
