Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells
10.13422/j.cnki.syfjx.20192124
- VernacularTitle: 薄荷醇对肝癌HepG2细胞增殖、迁移及IL-8，CXCL-12，VEGF表达的影响
- Author:
Xing-kui TAO
1
;
Xing-tao ZHANG
1
;
Hai-chao WANG
1
;
Hong DUAN
1
;
Jin CHENG
1
Author Information
1. Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, Suzhou 234000, China
- Publication Type:Research Article
- Keywords:
menthol;
cell proliferation;
cell migration;
HepG2 cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):60-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of menthol on proliferation, migration and expressions of interleukin-8(IL-8), C-X-C motif chemokine-12(CXCL-12) and vascular endothelial growth factor(VEGF) in hepatoma HepG2 cells in vitro, in order to elucidate the anti-liver cancer activity of menthol and relevant mechanisms. Method: Different concentrations of menthol (25, 50, 100, 200 μmol·L-1) and blank group were applied to Hepatoma HepG2 cells. Cell counting kit-8(CCK-8) and EDU were used to detect the proliferation effect of menthol on HepG2 cell. Transwell experiment was used to detect the migration effect of menthol on HepG2 cell. Real-time fluorescent quantitative polymerase chain reaction technique was used to detect the inflammatory chemokines IL-8 and CXCL-12 mRNA expression levels in HepG2 cell. Western blot was used to detect the expression level of VEGF in HepG2 cells treated with menthol. Result: Compare with the blank group, menthol (25, 50, 100, 200 μmol·L-1) significantly inhibited proliferation and migration of HepG2 cells in vitro. When the concentration of menthol was 100 μmol·L-1 microns, the inhibitory effect was significant (P<0.05). Menthol significantly inhibited the migration of HepG2 cells. The higher the concentration of menthol was, the less the number of cell migration was. Compared with the blank group, menthol (100, 200 μmol·L-1) significantly inhibited the expression levels of IL-8, CXCL-12 mRNA and VEGF protein in HepG2 cells (P<0.05), indicating that menthol is an anticancer agent by inhibiting inflammation and chemokines. Conclusion: Menthol has an inhibitory effect on the proliferation and migration of hepatoma HepG2 cells in vitro, and the potential anti-liver cancer mechanism might be related to the inhibition of IL-8, CXCL-12 and VEGF expressions in the cells. This conclusion provides the experimental basis for elucidating the effect of menthol against liver cancer.
