Effect of Tongfengning Serum Containing on Expression of Urate Transporter in HK-2 Induced by Uric Acid
10.13422/j.cnki.syfjx.20192139
- VernacularTitle: 痛风宁含药血清对尿酸诱导HK-2中尿酸盐转运体的影响
- Author:
Bao-lin LI
1
;
Jian-hui WANG
1
;
Tang-yan CAI
2
;
Jie-mei GUO
1
;
Fang-zhou TENG
1
;
Ya-ju ZHU
1
;
Jian-ping LIN
3
;
Xiao MAO
1
;
Yan XIAO
1
;
You-xin SU
1
Author Information
1. College of Traditional Chinese Medicine(TCM), College of Rehabilitation Medicine, Fujian University of TCM, Fuzhou 350122, China
2. Fuijian Health College, Fuzhou 350101, China
3. College of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China
- Publication Type:Research Article
- Keywords:
Tongfengning serum containing;
urate;
human renal tubular epithelial cells(HK-2);
urate transporter
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):53-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Study on the mechanism of Tongfengning in reducing serum uric acid from the perspective of renal urate transporter. Method: The human renal tubular epithelial cells(HK-2)was randomly divided into normal group, model group, Tongfengning low, medium and high dose group (7.65,15.3,30.6 g·kg-1) and benzbromarone group (50 μmo1·L-1),different culture media were given for intervention.HK-2 and cell supernatant were collected after 24 h of intervention. The expressions of urate transporter 1 (URAT1), glucose transporter 9 (GLUT9), organic anion transporter 1(OAT1), organic anion transporter 3(OAT3), and ATP-binding cassette superfamily G member 2 (ABCG2) protein and mRNA were detected in HK-2 of all groups by Western blot and Real-time PCR. Result: Compared with normal group, the expression of URAT1, GLUT9 protein and mRNA was significantly increased(P<0.01), while the expression of ABCG2 protein and mRNA was significantly decreased in model group (P<0.01). Compared with model group, the expression of URAT1, GLUT9 protein and mRNA in each dose of Tongfengning group and benzbromarone group were decreased (P<0.01), and each doses of Tongfengning group was superior to the benzbromarone group. The expression of ABCG2 protein and mRNA was increased in each dose of Tongfengning group (P<0.01). Compared with Tongfengning medium dose group, the expression of URAT1,GLUT9 protein and mRNA increased, while the expression of ABCG2 protein and mRNA decreased in the low and Tongfengning high dose groups (P<0.01). OAT1 and OAT3 were not expressed in all groups. Conclusion: Tongfengning can regulate the reabsorption and secretion of uric acid in renal tubules, promote the excretion of uric acid in kidney and reduce the level of serum uric acid by down-regulating the expression of URAT1, GLUT9 protein and mRNA in HK-2 and up-regulating the expression of ABCG2 protein and mRNA. It is suggested that the regulation of renal uric acid transporter protein may be one of the specific mechanisms of Tongfengning to reduce serum uric acid by promoting dampness and turbid removal. OAT1, OAT3 protein and mRNA were not expressed in HK-2 cultured in vitro.
