Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes
10.13422/j.cnki.syfjx.20192136
- VernacularTitle: 治疗糖尿病中药有效组分配伍比例的筛选与验证
- Author:
Xiao WANG
1
;
Xiao-bo QU
1
;
Bo LIU
2
;
Xin WANG
2
;
Lian-lian SONG
2
;
Hui-bo XU
2
Author Information
1. School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
2. Jilin Academy of Chinese Medical Sciences, Changchun 130012, China
- Publication Type:Research Article
- Keywords:
diabetic complication;
total alkaloids of Coptidis Rhizoma;
Notoginseng Radix et Rhizoma total saponins;
uniform design;
compatibility proportion
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):45-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the best compatibility proportion of total alkaloids of Coptidis Rhizoma and Notoginseng Radix et Rhizoma total saponins (HS composition)by uniform design and pharmacological model and to observe the effect on diabetic complications. Method: The total alkaloids of Coptidis Rhizoma and Notoginseng Radix et Rhizoma total saponins were used as the research objects, U6(62) table was choosed for grouping design.The content of triglyceride fasting blood-glucose (FBG), prothrombin time (PT) and active partial thromboplastin time (APTT) was chosen as index. The best dose ratio was obtained by multipleregression analysis. Rats with type 2 diabetes mellitus induced by high-fat diet combined streptozotocin were divided into blank group, model group, metformin group (150 mg·kg-1), HS composition group (total alkaloids of Coptidis Rhizoma 360 mg·kg-1+ Notoginseng Radix et Rhizoma total saponins 40 mg·kg-1). Rats were administered orally for 10 weeks.By observing the blood glucose, glucose tolerance,area under the curve (AUC), triglyceride (TG), high-density lipoprotein (HDL-C)and low-density lipoprotein (LDL-C), hemorheological indexes and pathological changes of pancreas, heart, kidney and retina in rats of each group, the effect of this composition on diabetic complications was verified. Result: Combination of 625 mg·kg-1 total alkaloids of Coptidis Rhizoma and 60 mg·kg-1 Notoginseng Radix et Rhizoma total saponins was the optimal dosage ratio of HS composition.The validation test showed that compared with blank group, the fasting blood glucose and lipid levels in the model group were significantly increased (P<0.01), and HDL-C was decreased (P<0.01).The hemorheology indexes of whole blood were significantly increased (P<0.01).Compared with model group, the FBG in metformin group were significantly reduced at week 6,8,10(P<0.05,P<0.01). Compared with model group,HS combination group 2, 4, 8, 10 weeks significantly lower fasting glucose levels(P<0.05,P<0.01), serum TG, LDL-C significantly lower values(P<0.05,P<0.01), HDL-C, HDL-C/LDL-C values were significantly increased (P<0.01), whole blood high shear viscosity and whole blood viscosity were significantly reduced (P<0.01).HS composition could reduce the blood sugar and lipid content, reduce the whole blood viscosity, and reduce the damage of pancreas, heart, kidney and retina. Conclusion: The optimum compatibility dose of HS composition have a good therapeutic effect on diabetic complication rats induced by high-fat diet and streptozotocin.
