Mechanism of Baoyuan Jiedu Decoction in Alleviating Muscle Atrophy in Apcmin/+ Cachexia Mice
10.13422/j.cnki.syfjx.20192102
- VernacularTitle: 保元解毒汤改善Apcmin/+恶病质模型肌肉萎缩的机制分析
- Author:
Xin ZONG
1
;
Ya-nan ZHANG
1
;
Hong-hua ZHANG
1
;
Xu-ming JI
2
Author Information
1. Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China
2. Basic Medical School, Zhejiang Chinese Medical University, Hangzhou 310053, China
- Publication Type:Research Article
- Keywords:
Apcmin/+;
cancer cachexia;
muscular atrophy;
Baoyuan Jiedu decoction;
uncoupling protein
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(21):19-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Baoyuan Jiedu decoction on serum interleukin-6 (IL-6) content, expression of muscle atrophy F-box 1(Atrogin-1), muscle ring finger-1 (MuRF-1), uncoupling proteins-2 (UCP-2), uncoupling proteins-3 (UCP-3) in Apcmin/+ mice, in order to explore the mechanism in improving muscle atrophy in cancer cachexia model. Method: The 14-week-old Apcmin/+ cachexia mice model was randomly divided into model group, Baoyuan Jiedu decoction group (23 g·kg-1) and megestrol group (0.024 g·kg-1). C57BL/6J mice were normal group, with 10 mice in each group, and given continuous intragastric administration for 12 weeks. The quality of gastrocnemius muscle and the transverse diameter of muscle fibers were measured. The content of IL-6 in serum of Apcmin/+ cachexia mice was detected by enzyme-linked immunosorbent assay (ELISA). The expressions of Atrogin-1, MuRF-1, UCP-2, UCP-3 mRNA and protein in gastrocnemius muscle were detected by Western blot and quantitative real-time fluorescence polymerase chain reaction (Real-time PCR). Result: Compared with the normal group, the weight of gastrocnemius muscle and the transverse diameter of fibers in the model group decreased significantly (P<0.05), while the concentration of IL-6 in serum and the expressions of Atrogin-1, MuRF-1, UCP-2 and UCP-3 in gastrocnemius muscle of mice increased significantly (P<0.05). Compared with the model group, the weight of gastrocnemius muscle, the expression of UCP-3 and protein in the treated group increased significantly (P<0.05). The transverse diameter of muscle fibers increased significantly, whereas the concentration of IL-6 in serum and the expressions of Atrogin-1, MuRF-1, UCP-2 and UCP-3 in gastrocnemius muscle of mice decreased significantly (P<0.05). Conclusion: Reduction of the concentration of IL-6 in serum and the down-regulation of the expressions of Atrogin-1, MuRF-1, UCP-2 and UCP-3 genes may be the possible mechanism of Baoyuan Jiedu decoction in alleviating muscle atrophy in Apcmin/+ cachexia mice model.
