Chemical Constitutes of Cardiac Glycosides in Scurrula parasitica and Its Nerium indicum by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20192211
- VernacularTitle: 基于UPLC-Q-TOF-MS/MS技术的红花寄生及其寄主夹竹桃强心苷成分相关性分析
- Author:
Zi-shu CHAI
1
;
Ren-yuan LIU
1
;
Li-zhang LI
1
;
Fei-ying HUANG
1
;
Ben-wei SU
2
;
Kai-xin ZHU
2
;
Yong-hua LI
1
Author Information
1. Guangxi University of Chinese Medicine, Nanning 530200, China
2. Qinzhou Institute of Traditional Chinese Medicine, Qinzhou 535000, China
- Publication Type:Research Article
- Keywords:
Scurrula parasitica;
Nerium ndicum;
cardiac glycoside;
host;
Osmanthus fragrans
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):138-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for identifying cardiac glycosides in Scurrula parasitica and its Nerium indicum host by UPLC-Q-TOF-MS/MS. With safflower parasitoids with sweet-scented osmanthus trees as the host and their host osmanthus tree samples used for control, the chemical constituents of the cardiac glycosides were identified by comparison between the cardiac glycoside reference substances and literatures, so as to analyze the correlation between the safflower parasitoid and its host oleander glycoside components,and evaluate the host' s impact on the quality of Taxilli Herba. Method: Samples of S. parasitica (parasitic on N. indicum and Osmanthus fragrans),N. indicum and O. fragrans were collected. Samples of S. parasitica and its O. fragrans host were taken for control. All of the samples were extracted through ultrasonic extraction with 70%ethanol. ACQUITY UPLC HSS T3 C18(2.1 mm×100 mm,1.8 μm) column was adopted with mobile phase A comprising 0.1%formic acid water and mobile phase B comprising acetonitrile for gradient elution. The sample size was 0.5 μL. The flow rate was 0.6 mL·min-1. The column temperature was maintained at 40℃. MassLynx V4.1 software was used to analyze the data. Identification and correlation of chemical constitute of cardiac glycosides in S. parasitica and its N. indicum host were performed through analysis on cardiac glycosides reference substances,relevant literatures,elemental composition of compounds and positive and negative ion mode mass spectrometry data. Result: A total of 26 compounds of cardiac glycoside were identified,including 25 compounds of cardiac glycoside from N. indicum host,and 5 compounds of cardiac glycoside from S. parasitic(parasitic on N. indicum). none of cardiac glycosides were found in S. parasitica (parasitic on O. fragrans ) and its O. fragrans host. Conclusion: It was rapid,accurate and comprehensive to identify cardiac glycosides in S. parasitica (parasitic on N. indicum) and its N. indicum host by UPLC-Q-TOF-MS/MS. S. parasitica itself does not contain cardiac glycosides,its host may impact the quality of S. parasitica by delivering cardiac glycosides, a kind of its characteristic compound.
