Analysis of Pharmacokinetic Behavior of Five Components in Qingkailing (Lyophilized) for Injection in Normal Rats and Cerebral Ischemia Rats by UPLC-MS/MS
10.13422/j.cnki.syfjx.20191452
- VernacularTitle: UPLC-MS/MS分析注射用清开灵(冻干)中5种成分在正常大鼠和脑缺血大鼠体内的药代动力学行为
- Author:
Xue LIU
1
;
Ju SU
1
;
Peng DU
2
;
Wen-li YAO
1
;
Qing-bo YANG
3
;
Yu-mei LU
3
;
Lin-jing WU
1
;
Feng JIANG
1
;
Xiang-chun SHEN
1
;
Qian-li XU
1
;
Ling TAO
1
;
Xiang-jun MAO
1
Author Information
1. Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicinal Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Joint Key Laboratory of Guizhou Medical University-Guiyang City, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, China
2. The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China
3. Guizhou Yibai Pharmaceutical Co. Ltd., Guiyang 550008, China
- Publication Type:Research Article
- Keywords:
Qingkailing (lyophilized) for injection;
cerebral ischemia model;
pharmacokinetics;
rutin;
isorhamnetin;
endogenous bile acids;
intraperitoneal injection
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):86-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a UPLC-MS/MS analysis method for determination of baicalin, geniposide, chlorogenic acid, cholic acid and hyodeoxycholic acid in Qingkailing (lyophilized) for injection in rat plasma, and to investigate the pharmacokinetic behavior of this preparation in normal and cerebral ischemic rats. Method: Rats were randomly divided into normal group and cerebral ischemia model group. The rat model of cerebral ischemia was established by suture embolization. The rats were given by intraperitoneal injection, and normal saline was used as the solvent. Blood samples were taken at the corresponding time points. After treatment, UPLC-MS/MS was used to determine the blood concentration of five components. The main detection conditions were mobile phase of 0.1%formic acid aqueous solution-acetonitrile for gradient elution (0-0.25 min, 90%A; 0.25-1 min, 90%-75%A; 1-2 min, 75%-50%A; 2-2.6 min, 50%-45%A; 2.6-2.65 min, 45%-90%A; 2.65-4.0 min, 90%A), the flow rate of 0.4 mL·min-1, the column temperature at 40℃, electrospray ionization under negative ion mode. The pharmacokinetic parameters were fitted and the bioavailability was calculated, the differences of treatment process of five components from Qingkailing (lyophilized) for injection in normal and cerebral ischemic rats were analyzed. Result: Compared with the normal group, the area under the curve (AUC0-t) of geniposide in rats from cerebral ischemia model group decreased significantly after intraperitoneal injection of Qingkailing (lyophilized) for injection (P<0.05), and the time to peak (Tmax) of chlorogenic acid in rats from cerebral ischemia model group was significantly earlier than that in the normal group (P<0.01). Pharmacokinetic parameters of baicalin, cholic acid and hyodeoxycholic acid had no significant difference between these 2 groups. Conclusion: Qingkailing (lyophilized) for injection has a certain difference in the treatment process between normal and cerebral ischemic rats, which has certain guiding significance for the clinical treatment of cerebral ischemic diseases with this preparation.
