Analysis of Mechanism of Artesunate in Treatment of Liver Cancer Based on Metabolomics
10.13422/j.cnki.syfjx.20191548
- VernacularTitle: 基于代谢组学分析青蒿琥酯治疗肝癌的作用机制
- Author:
Hui-guo WANG
1
;
Si-yao WANG
1
;
Xin-long HAN
1
;
Yu-tong LI
1
;
Li-xia YUAN
2
;
Xiao-mei TAN
2
;
Qing-fa TANG
2
;
Xue-feng XING
2
;
Fei-long CHEN
2
;
Ya-lei YIN
3
;
Ling TANG
2
Author Information
1. School of Life Science and Technology, Dalian University, Dalian 116622, China
2. Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology, Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
3. Scientific Research Center for Translational Medicine, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
- Publication Type:Research Article
- Keywords:
artesunate;
metabolomics;
liver cancer;
Huh7;
SMMC-7721;
amino acid metabolism;
energy metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):78-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study on the antitumor mechanism of artesunate in the treatment of liver cancer based on gas chromatography-mass spectrometry (GC-MS). Method: CellTiter-Glo® Luminescent Cell Viability Assay was used to detect activity of artesunate with different concentrations (0, 12.5, 25, 50, 100 μmol·L-1) on human liver cancer Huh7, SMMC-7721 cells for 24, 48, 72 h. GC-MS was employed to analyze the changes of metabolites of artesunate in two kinds of hepatoma cells (Huh7, SMMC-7721) for 24 h. The data was preprocessed by Postrun Analysis 4.41 workstation. Partial least squares-discriminant analysis (PLS-DA) was used to analyze two sets of differential metabolites and to analyze metabolic pathways of differential metabolites based on MetaboAnalyst 3.0 software. Result: Compared with the normal group, after two kinds of liver cancer cells was treated by artesunate, a total of 39 identical metabolites in the cells have undergone significant changes, which were mainly related to five metabolic pathways,including biosynthesis of aminoacyl-transfer RNA (tRNA), metabolism of alanine, aspartic acid and glutamic acid, metabolism of glycine, serine and threonine, metabolism of arginine and proline, metabolism of glutathione. Conclusion: Artesunate (12.5-100 μmol·L-1) can inhibit the growth of liver cancer cells (Huh7, SMMC-7721), it mainly involves five metabolic pathways, which may be the pathway of artesunate against liver cancer.
