Evaluation of Rat Model with Spleen Deficiency and Dampness Stagnancy Based on Bioelectrical Impedance
10.13422/j.cnki.syfjx.20192138
- VernacularTitle: 基于生物电阻抗评价大鼠脾虚水湿内停模型
- Author:
Wei LI
1
;
Ran CHEN
1
;
Mei-feng OU
1
;
Sha-sha JIANG
1
;
Sheng-nan FAN
1
;
Yun-lai WANG
1
;
Fan XU
1
Author Information
1. School of Pharmacy, Key Laboratory of Chinese Medicine Formula of Anhui Province, Anhui University of Chinese Medicine, Hefei 230012, China
- Publication Type:Research Article
- Keywords:
spleen deficiency and dampness stagnancy;
bioelectrical impedance;
experimental animal body composition determination analyzer;
water liquid metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):49-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the model with spleen deficiency and dampness stagnancy by bioelectrical impedance analysis (BIA) and traditional indicators. Method: The forty rats were divided into blank group and model group, with 20 rats in each group. The rats in the blank group were fed with normal feed, the rats in model group were prepared with the spleen deficiency and dampness stagnancy model for 14 days. Observe the general condition of the rats, measure the water content of the feces in the dry method, measure the water load index by weighing method, and detect the urinary D-xylose excretion total protein (TP), albumin (Alb) content, by enzyme-linked immunosorbent assay (ELISA). Western blot analysis of renal aquaporin 1 (AQP1) content, and the use of experimental animal body composition analyzer to determine the total water content (TBW), extracellular fluid (ECF), intracellular fluid (ICF), fat mass (FM), free fat mass (FFM) and body mass bioelectrical impedance index such as body mass index (BMI). Result: Compared with blank group, the rats in model group lost weight, gradually loose stools occasionally, the anus temperature was basically unchanged, body mass, D-xylose excretion, water load index, TP and Alb content decreased (P<0.01). The feces contained water,the rate and the content of AQP1 in the renal pulp were increased (P<0.01). The TBW, ECF and FFM were increased, and the ICF, FM and BMI were decreased (P<0.01). Conclusion: Rats with spleen deficiency and dampness stagnancy induced a combination of factors such as diet and excessive fatigue. The bioelectrical impedance method can be more intuitive and comprehensive.
