Mechanism of Dichroa Alkali Salt Inducing Vomiting Based on Pica in Mice
10.13422/j.cnki.syfjx.20191722
- VernacularTitle: 基于小鼠异食癖模型的常山碱盐呕吐机制
- Author:
Li-na MA
1
;
Jian LI
1
;
Si-di LI
1
;
Bao-qiang DAI
1
;
Guang-ping ZHANG
1
;
Hong-ping HOU
1
;
Ping SU
1
;
Feng-yu WANG
1
;
Zu-guang YE
1
Author Information
1. Post-doctoral Scientific Research Center, Institute of Chinese Materia Medical, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
dichroa alkali salt;
vomiting;
pica in mice;
compatibility;
antiemetic drug
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(22):34-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the possible mechanism of dichroa alkali salt (DAS) in inducing vomiting. Method: Mice pica model was used to observe the antagonistic effect of the three different kinds of antiemetic drugs[dopamine receptor antagonist metoclopramide, 5-hydroxytryptamine 3 (5-HT3) receptor antagonist ondansetron and neurokinin-1 receptor antagonist aprepitant] on body mass, food intake, kaolin consumption, diarrhea and death induced by DAS to preliminarily clarify the possible pathogenic pathway of DAS. Then, the expression of 5-HT and substance P(SP) in ileum and medulla of mice induced by DAS alone at different time points was detected by enzyme-linked immunosorbent assay to confirm whether DAS could affect the changes of these two neurotransmitters. Result: After treatment with ondansetron and aprepitant, DAS-induced reduction in food intake of mice was significantly improved on the 4th day after continuous administration and on the 1st day after drug administration (P<0.01), since the 3rd day after administration, DAS-induced body mass loss of mice was significantly improved (P<0.01), and DAS-induced diarrhea and mortality in mice were significantly reduced, while DAS-induced pica in mice was effectively antagonized. However, metoclopramide did not significantly improve the above indicators. Further detection of vomiting-related neurotransmitters found that compared with the blank group, the expressions of 5-HT at 4 h, 12 h and SP at 48 h after treatment with DAS were significantly increased in ileum and medulla of mice. Conclusion: The mice pica model can be used to effectively characterize DAS-induced vomiting. DAS-induced pica in mice may be associated with the increase of 5-HT and SP in ileum and medulla. Ondansetron and aprepitant can effectively antagonize DAS-induced pica in mice.
