Analysis on Mechanism of Shema Zhichuan Liquid in Treating Asthma Based on Network Pharmacology
10.13422/j.cnki.syfjx.20191461
- VernacularTitle: 基于网络药理学探索射麻止喘液治疗哮喘的作用机制
- Author:
Xiu-fang HUANG
1
;
Yun-ji GAO
2
;
Gang LIAO
2
;
Hui-ting HUANG
3
;
Shao-feng ZHAN
3
;
Yan-ni LAI
1
;
Xiao-hong LIU
3
Author Information
1. The First Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
2. Shenzhen Hospital of Guangzhou University of Chinese Medicine, Shenzhen 518000, China
3. Guangdong Provincial Inheritance Studio of Famous Traditional Chinese Medicine of LIU Xiao-hong, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
network pharmacology;
Shema Zhichuan liquid;
asthma;
bioinformatics;
gene ontology;
pathway enrichment;
protein-protein interaction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(23):187-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the potential mechanism of Shema Zhichuan liquid in treatment of asthma by network pharmacology. Method: Bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine (TCM), systematic pharmacological database and analysis platform of TCM were employed to find the components in Shema Zhichuan liquid and their targets, and asthma-related genes were obtained from the comparative toxicogenomics database (CTD). The data set of Shema Zhichuan liquid-gene and asthma-gene were imported into the Draw Venn Diagram for intersection analysis. The obtained data set of Shema Zhichuan liquid-asthma-gene was imported into String 11.0 for protein-protein interaction (PPI) analysis, and was visualized by Cytoscape 3.6.1, and further important modules were analyzed with MCODE. DAVID 6.8 was used to analyze pathway enrichment and biological process of Shema Zhichuan liquid-asthma-gene. Result: A total of 399 components and 2 099 potential targets were obtained from Shema Zhichuan liquid, 98 asthma-related targets were retrieved, 45 common genes and 16 hub genes were screened, including transforming growth factor-β1(TGF-β1), heme oxygenase-1 (HMOX1), interleukin-4 (IL-4), etc. Enrichment analysis showed that the common biological processes of Shema Zhichuan liquid and asthma were related to inflammation, contraction and remodeling of airway, cell proliferation and apoptosis, etc. The common biological pathways mainly included tumor necrosis factor (TNF) signaling pathway, receptor with high affinity for immunoglobulin E (Fc epsilon RI) signaling pathway, nuclear transcription factor-kappa B (NF-κB) signaling pathway, nucleotide binding oligomerization domain (NOD)-like receptor signaling pathway and so on. Conclusion: Shema Zhichuan liquid serves as a multi-target, multi-pathway treatment for asthma, which can provide a reference for the further research and clinical application of this preparation.
