Isolation and Identification of Chemical Constituents from Rhizomes of Dendropanax proteus
10.13422/j.cnki.syfjx.20192349
- VernacularTitle: 变叶树参化学成分的分离与鉴定
- Author:
Jian ZHOU
1
;
Yu-hua LI
1
;
Ying ZHOU
1
;
Yan-ni LYU
1
;
Hong-wei PENG
1
;
Ming-jin JIANG
1
;
Song TAO
1
;
Jin-hua WEN
1
;
Xiao-hua WEI
1
Author Information
1. The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
- Publication Type:Research Article
- Keywords:
Dendropanax proteus rhizomes;
95% ethanol extract;
isolation and purification;
structural elucidation;
lignans;
phenolic acids;
anti-inflammatory activity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(23):128-132
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents from 95% ethanol extract of Dendropanax proteus rhizomes and their anti-inflammatory activities were investigated. These compounds in 95% ethanol extract of D. proteus rhizomes were isolated and purified by silica gel column chromatography, medium-pressure liquid chromatography, preparative liquid chromatography, etc. Their structures were elucidated based on the spectral data and physicochemical properties. All the compounds were tested for their ability to inhibit lipopolysaccharide (LPS) induced nitric oxide production in the murine microglia BV2 cell line. Nine compounds were isolated from the ethyl acetate fraction of 95% ethanol extract of D. proteus rhizomes, and identified as (-)-syringaresinol (1), (+)-(7S,8S)-1',4-dihydroxy-3,3',5'-trimethoxy-7',8,9'-trinor-8,4'-oxyneoligna-7,9-diol (2), erythro-guaiacylglycerol-β-O-4'-coniferyl ether (3), threo-guaiacylglycerol-β-O-4'-coniferyl ether (4), coniferyl alcohol (5), 7-O-ethylguaiacylglycerol (6), vanillin (7), syringaldehyde (8), and excoecanol B (9). Compounds 2 and 4 showed neuritis inhibitory activity against microglial inflammation factor, their half inhibitory concentrations (IC50) were 5.85, 7.29 μmol ·L-1, respectively. Compounds 1-6,8-9 are isolated from this plant for the first time, compounds 2 and 4 exhibit the potent inhibitory activity.
