Effect of Modified Erchentang on β2AR/β-arrestin2 Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease
10.13422/j.cnki.syfjx.20192337
- VernacularTitle: 二陈汤加味对慢性阻塞性肺疾病大鼠β2AR/β-arrestin2信号通路的影响
- Author:
Wen-ying XIE
1
;
Jun-yue WANG
1
;
Yong-sheng BAO
1
;
Li-zhi SHANG
1
;
Ke WU
1
;
Liang LI
1
;
Xiao-yan WANG
1
;
Xiao-hui CHEN
1
;
Xiao-fang LI
1
;
Qian-hong SONG
1
Author Information
1. Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
modified Erchentang;
chronic obstructive pulmonary disease;
β2 adrenergicreceptor;
arrestin beta 2;
interleukin-17
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(23):34-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of modified Erchentang on the signal pathway of β2 adrenergicreceptor(β2AR)/arrestin beta 2(β-arrestin2) in rats with chronic obstructive pulmonary disease (COPD), and the expression of interleukin-17(IL-17) in serum, lung homogenate and bronchoalveolar lavage fluid. Method: Seventy SD rats were randomly divided into seven groups:normal group, model group, modified Erchentang with high, medium and low doses (40, 20, 10 g · kg-1 · d-1), Xiaokechuan group (5 g · kg-1 · d-1), modified Erchentang group (5 g · kg-1 · d-1), 10 rats in each group. The rat model of COPD was established by smoking and lipopolysaccharide (LPS) intratracheal drip. After successful modeling, the treatment group was given intragastric administration, while the normal group and the model group were given the same amount of saline. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-17 in serum, lung homogenate and bronchoalveolar lavage fluid of rats. Real-time fluorescence quantitative PCR (Real-time PCR) was used to detect the expression of β2AR gene. Western blot was used to detect the expression of β2AR protein in lung tissue. The expression of β2AR and β-arrestin2 in lung tissue was detected by immunohistochemistry. Result: Compared with the normal group, the expression of β2AR protein in lung tissue of model group was significantly decreased(P<0.01). Compared with model group, the expression of β2AR protein in lung tissue was significantly increased(P<0.01), and the middle dose group of modified Erchentang was different from other groups (P<0.05). Compared with normal group, the expression of β2AR in model group was significantly lower(P<0.01), compared with model group, the expression of β2AR in high, medium and low dose group, Xiaokechuan group and modified Erchentang group was significantly higher(P<0.01). The middle dosage group of modified Erchentang was significantly higher than other groups(P<0.01). Compared with normal group, the serum level of IL-17 in the model group was significantly higher(P<0.01). Compared with model group, the serum level of IL-17 in each group was inhibited to a certain extent, especially in the middle dose group of modified Erchentang (P<0.05). Conclusion: Modified Erchentang may increase the expression of β2AR and β-arrestin2 and decrease the content of IL-17 in order to resist inflammation and improve pulmonary function in COPD rats.
