Effect of Modified Erchentang on GATA3 and T-bet mRNA Expressions in Lung Tissue of Chronic Obstructive Pulmonary Disease Rats
10.13422/j.cnki.syfjx.20191701
- VernacularTitle: 二陈汤加味对慢性阻塞性肺疾病大鼠肺组织GATA3，T-bet mRNA表达的影响
- Author:
Yong-sheng BAO
1
;
Wen-ying XIE
1
;
Jun-yue WANG
1
;
Li-zhi SHANG
1
;
Ke WU
1
;
Liang LI
1
;
Xiao-yan WANG
1
;
Xiao-hui CHEN
1
;
Xiao-fang LI
1
;
Qian-hong SONG
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
Erchentang;
chronic obstructive pulmonary disease;
GATA-binding protein-3(GATA3);
T-box expressed in T cells(T-bet);
interleukin-10;
interleukin-12
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(23):19-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of modified Erchentang on GATA-binding protein-3(GATA3) and T-box expressed in T cells(T-bet) in lung tissue of rats with chronic obstructive pulmonary disease (COPD). Method: Seventy SD rats were randomly divided into seven groups, namely normal group, model group, low, medium and high-dose modified Erchentang group(5,10,20 g ·kg-1), Xiaokechuan group(5 g ·kg-1) and Erchentang group(5 g ·kg-1), with 10 in each group. The rat model of COPD was established by smoking combined with intratracheal dripping of lipopolysaccharide (LPS). After successful modeling, the treatment group was given intragastric administration, and the normal group and the model group were given intragastric administration of equal volume of saline. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentrations of interleukin-10 (IL-10) and interleukin-12 (IL-12) in rat serum. The expressions of GATA3 and T-bet were detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). The expressions of GATA3 and T-bet in lung tissue were detected by immunohistochemistry (IHC). Result: Compared with the control group, the serum levels of IL-10 in the model group was significantly decreased, while the IL-12 level was significantly increased (P<0.01). The protein and gene expressions of GATA3 in lung tissue were significantly decreased, whereas the expression of T-bet was significantly increased (P<0.01). Compared with the model group, the serum level of IL-10 in each treatment group was increased to varying degrees, while the level of IL-12 was decreased to varying degrees (P<0.05). The mRNA and protein expressions of GATA3 in lung tissue of rats in each treatment group were increased significantly, while T-bet was inhibited (P<0.05). Conclusion: Modified Erchentang may reduce the inflammation of lung tissue and improve lung function in COPD rats by reducing IL-12, increasing the content of IL-10, inhibiting the protein and gene expressions of T-bet, and stimulating the protein and gene expressions of GATA3.
