Effect of Oxymatrine in Inhibiting Proliferation and Migration of HT-29 Cells Induced by Insulin
10.13422/j.cnki.syfjx.20192022
- VernacularTitle: 氧化苦参碱抑制胰岛素诱导人结肠癌HT-29细胞的增殖及迁移作用
- Author:
Di PAN
1
;
Wen ZHANG
1
;
Rong ZHANG
1
;
Shi-quan GAN
1
;
Yan CHEN
1
;
Nen-ling ZHANG
1
;
Yi-ni XU
1
;
Xiang-chun SHEN
1
Author Information
1. Guizhou Province Natural Medicinal Resources High-Efficacy Application Engineering Center, Guiyang 550025, China
- Publication Type:Research Article
- Keywords:
HT-29 cell;
oxymatrine;
insulin;
cell proliferation;
cell migration
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):36-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of oxymatrine (OMT) on the proliferation and migration of human colon cancer cell line HT-29 under Type Ⅱ diabetes environment by co-culturing HT-29 with insulin to simulate hyperinsulinemia. Method:The effect of OMT (2, 4, 8 mmol·L-1) on insulin-induced proliferation of HT-29 was detected by methyl thiazolyl tetrazolium (MTT) assay and cloning assay. The morphology change and cell migration were evaluated under microscope and by wound healing assay. The Annexin V/propidium iodide(PI) assay was used to detect the change of insulin-induced HT-29 cell cycle and apoptosis. Western blot was performed to validate the expression of cell cycle-related protein and cell migration protein. Result:Insulin significantly increased growth of HT-29 (P<0.05). Compared with insulin group, OMT with 2, 4, 8 mmol·L-1 showed a significant inhibitory effect in this model (P<0.05). In addition, OMT blocked HT-29 cell cycle in G0/G1 phase (P<0.05), and showed a slight apoptotic effect. Western blot showed that the down-regulation of Cyclin D1, CDK4 and the up-regulation of p27 by OMT might involve the growth inhibition mechanism. Furthermore, OMT reduced the migration of insulin-induced HT-29 according to wound healing assay(P<0.05). Decreased Vimentin (P<0.05)and increased E-cadherin(P<0.05)might be correlated with the migration restrain. Conclusion:OMT can inhibit the proliferation and migration of insulin-induced HT-29 cells. The changes of cell cycle and migration related proteins may be correlated with the mechanism.
