Effect of Yiqi Huoxue Recipe on Oxidative Stress Response in Rats with Cerebral Ischemia Injury Based on Nrf2/HO-1 Signaling Pathway
10.13422/j.cnki.syfjx.20192437
- VernacularTitle: 基于Nrf2/HO-1信号通路探讨益气活血方对脑缺血损伤大鼠氧化应激反应的影响
- Author:
Yuan-yuan GU
1
;
Shu-xiang ZHANG
1
;
Zhong-guang ZHOU
1
;
Xu LIU
1
;
Ming TIAN
1
;
Hui LIANG
1
;
Yan-qi SHANG
1
Author Information
1. Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
- Publication Type:Research Article
- Keywords:
Yiqi Huoxue recipe;
cerebral ischemic injury;
oxidative stress;
haem oxygenase-1(HO-1);
nuclear factor E2-related factor 2(Nrf2)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(24):30-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of Yiqi Huoxue recipe on rats with cerebral ischemia injury by using oxidative stress injury as an entry point. Method:SD rats were randomly divided into model group, sham operation group, nimodipine group (20 mg·kg-1), Yiqi Huoxue recipe high, medium and low dose group (2.916,1.458,0.729 g·kg-1). After 14 days of stomach, acute cerebral ischemic injury model was established by ligation of bilateral common carotid arteries. Ultrasound of synapse was observed by transmission electron microscopy. Total superoxide dismutase (T-SOD) and dialdehyde (MDA), lactate dehydrogenase (LDH), glutathione peroxidase (GSH-Px), horizontal adenine nucleoside triphosphate (ATP) levels were detected by biochemical method. Western blot and Real-time PCR was used to determine the expression of heme oxygenase-1(HO-1) and nuclear factor E2-related factor 2 (Nrf2) protein and mRNA in the ischemic cortex of rats. Result:Transmission electron microscopy showed that Yiqi Huoxue recipe had a significant improvement on the degree of cerebral ischemic injury. Compared with sham operation group, MDA levels in the brain homogenate of model group increased significantly, T-SOD and GSH-Px levels were significantly decreased (P<0.01). The activity of LDH, Na+-K+-ATP ase, Ca2+-Mg2+-ATP ase and ATP was significantly decreased (P<0.05,P<0.01). The levels of HO-1 and Nrf2 protein and the expression of HO-1 and Nrf2 mRNA in brain tissue were significantly decreased (P<0.01). Compared with model group, the medium and high dose group of Yiqi Huoxue recipe significantly decreased MDA content in brain tissue and increased the levels of T-SOD and GSH-Px(P<0.05,P<0.01). Yiqi Huoxue recipe high dose group can significantly increase LDH, Na+-K+-ATP ase, Ca2+-Mg2+-ATP ase and total ATP activity(P<0.05,P<0.01). The contents of HO-1 and Nrf2 protein in the brain of rats with middle and high-dose Yiqihuoxue recipe group were significantly increased(P<0.05,P<0.01). The expressions of HO-1 and Nrf2 mRNA in high-dose Yiqi Huoxue recipe group were significantly increased(P<0.05,P<0.01). Conclusion:Yiqi Huoxue recipe may protect against cerebral ischemic injury by inhibiting oxidative stress through Nrf2/HO-1 signaling pathway.
