Effect of PEG400 Concentration in Receiving Solution on Drug Transdermal Test in Vitro
10.13422/j.cnki.syfjx.20181803
- VernacularTitle: 接受液中聚乙二醇400体积分数对药物体外透皮试验的影响
- Author:
Zi-xuan DU
1
;
Yi-bo WANG
1
;
Li-li GAO
1
;
Xing-bin YIN
1
;
Chang-hai QU
1
;
Xin FENG
2
;
Jian NI
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
2. Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
- Publication Type:Research Article
- Keywords:
receiving liquid;
polyethylene glycol 400;
in vitro transdermal test;
scanning electron microscopy;
phosphate buffer solution;
5-fluorouracil
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):196-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of varying concentrations of polyethylene glycol(PEG)400 in receiving solution on in vitro transdermal test of drugs. Method: 5-Fluorouracil(5-FU) was selected as a model drug,by preparing different concentrations of PEG400-phosphate buffer solution(PBS) as the receiving solution,the receiving chamber did not add drug,the excised rat skins were treated with various additives for 12 h,then replaced by PBS and added the saturated model drug into the donor compartment to determine the transdermal parameters of the drug.Meanwhile,scanning electron microscopy(SEM) was employed to monitor the effect of PEG400 with different concentration on the stratum corneum of rat skin. Result: The 10%,15% and 40% PEG400-PBS groups had no significant effect on in vitro transdermal absorption parameters of the 5-FU.The steady transdermal rate and cumulative penetration rate of the drug in 20% and 30% PEG400-PBS groups were significantly higher than that in the PBS group(P<0.01,P<0.05).SEM indicated that wrinkle of the intact rat skin gradually disappeared and a number of flakes were desquamated from the skin when the concentration of PEG400 was above 20% in receiving solution.Meanwhile,30% PEG400-PBS group and 40% PEG400-PBS group were extremely wrinkled. Conclusion: In the rat skin transdermal test,the concentration of PEG400 in receiving solution should be controlled below 20%.
