Quality Evaluation of Andrographis Herba Based On Electronic-eye Technique
10.13422/j.cnki.syfjx.20181702
- VernacularTitle: 基于电子眼技术的穿心莲质量评价
- Author:
Xiao ZHANG
1
;
Hong-wei WU
1
;
Xian-kuo YU
1
;
Ya-qi LU
1
;
Han-yan LUO
1
;
Hong-jun YANG
1
;
Meng-ying XU
1
;
Ri-xin GUO
1
;
Zhi-yong LI
2
;
Li-ying TANG
1
;
Zhu-ju WANG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections, Jiangxi Qingfeng Pharmaceutical Co. Ltd., Ganzhou 341000, China
- Publication Type:Research Article
- Keywords:
Andrographis Herba;
electronic-eye;
color values;
diterpene lactones;
correlation analysis;
principal component analysis;
content determination
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):189-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Taking electronic-eye (visual analyzer) technique,based on the powder color of Andrographis Herba,to investigate the applicability of electronic-eye technique and evaluate the quality of Andrographis Herba with different commercial specifications. Method: HPLC was employed to determine contents of andrographolide,dehydroandrographolide,14-deoxyandrographolide,neoandrographolide in 50 batches of Andrographis Herba with different commercial specifications(stems,leaves and aerial parts).Color of these samples were measured by electronic-eye technique.The data were analyzed by principal component analysis(PCA) and Pearson correlation analysis.The ability of electronic-eye to distinguish the different commercial specifications of Andrographis Herba was investigated and the correlation of chroma space system parameters (L*,a*,b*) with active components was investigated. Result: There was remarkable difference in contents of 4 diterpenoids in Andrographis Herba from different parts,their contents in leaves was the highest,followed by the aerial parts(mixture of stems and leaves),and their contents in stems was the lowest.The results of PCA was divided into two classes,namely the stem part,leaf and aerial parts,indicating that electronic-eye could be used to distinguish the quality of Andrographis Herba.The correlation results showed that there were significant negative correlation(P<0.01) between L*(lightness value) and the contents of andrographolide,dehydroandrographolide,14-deoxyandrographolide,neoandrographolide and the total content of these 4 components.In addition,L* of samples that did not conform to the lower limit of determination in the 2015 edition of Chinese Pharmacopeia was ≥ 69.5,and the L* of more than 90% of the samples in accordance with the requirements was <69.5. Conclusion: Electronic-eye technique provides a new method and idea for the quality evaluation of Andrographis Herba.
