Effect of Decocting Containers on Content of Sinapine and HPLC Specific Chromatograms of Sinapis Semen Decoction
10.13422/j.cnki.syfjx.20182303
- VernacularTitle: 煎药器皿对白芥子煎液中芥子碱含量及其特征图谱的影响
- Author:
Gang-yan YU
1
;
Yu-tao XUE
1
;
Li TAN
1
;
Xiang-long YU
1
;
Yu-jia MO
1
;
Ju-yuan LUO
1
;
Yang LU
1
;
Shou-ying DU
1
;
Jie BAI
1
;
Peng-yue LI
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Sinapis Semen;
decocting containers;
specific chromatograms;
sinapine thiocyanate;
similarity;
decoction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):183-188
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the effect of four kinds of decocting containers on the content of sinapine and the HPLC specific chromatograms of Sinapis Semen decoction,so as to optimize decocting container for the development of classical formulas. Method: Selecting four kinds of decoction vessels,named traditional casserole,ceramic pot,round-bottom flask and stainless-steel pot as the research object,the content of sinapine in Sinapis Semen decoction and its HPLC specific chromatograms were used as indexes to investigate the influence of different decoction vessels on the decoction.Similarity evaluation of specific chromatograms was performed by the "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine"(edition of 2004A). Result: The contents of sinapine in the decoction prepared by traditional casserole,ceramic pot,round-bottom flask and stainless-steel pot were 0.04%,0.07%,0.84% and 0.97%,respectively.Compared with specific chromatograms of the decoction prepared by traditional casserole,the similarities of specific chromatograms of the decoction prepared by ceramic pot,round-bottom flask and stainless-steel pot were 0.98,0.82 and 0.68,respectively.Compared with specific chromatograms of the decoction prepared by ceramic pot,the similarities of specific chromatograms of the decoction prepared by round-bottom flask and stainless-steel pot were 0.79 and 0.62,respectively.Compared with specific chromatograms of the decoction prepared by round-bottom flask,the similarity of specific chromatograms of the decoction prepared by stainless-steel pot was 0.97. Conclusion: The content of sinapine and HPLC specific chromatograms of Sinapis Semen decoction obtained from different decocting containers are quite different.
