Investigation of Physicochemical Properties and Determination Methods of Liposomal Entrapment Efficiency of Dichroa Alkali Hydrochloride
10.13422/j.cnki.syfjx.20182306
- VernacularTitle: 盐酸常山碱的理化性质及其脂质体包封率的测定方法考察
- Author:
Feng-yu WANG
1
;
Bao-xian ZHANG
1
;
Guang-ping ZHANG
1
;
Qi-xia NIE
1
;
Chen ZANG
1
;
Li-na MA
1
;
Ping SU
1
;
Hong-ping HOU
1
;
Yi-fei YANG
1
;
Zhong-xiu ZHANG
1
;
Meng MA
1
;
Zu-guang YE
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
dichroa alkali hydrochloride;
liposomes;
oil-water partition coefficient;
stability;
encapsulation efficiency;
microcolumn centrifugation method;
ammonium sulfate gradient method
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):177-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study on the physical and chemical properties of dichroa alkali hydrochloride and to establish a method for the determination of entrapment efficiency of dichroa alkali hydrochloride liposomes. Method: HPLC was used to determine the content of dichroa alkali hydrochloride with mobile phase of acetonitrile-water-triethylamine-glacial acetic acid(9:91:0.35:0.75) and detection wavelength at 265 nm.The oil-water partition coefficient of this compound in different pH range was measured by shake flask method.The stability of the dichroa alkali hydrochloride in phosphate buffer solution with different pH after sterilization at 125℃ for 30 min was investigated.Ammonium sulfate gradient method was used to prepare dichroa alkali hydrochloride liposomes.The microcolumn was prepared by dextran gel and cation exchange resin,respectively.Then the free drug and liposome were separated by centrifugation,the drug content was measured,and the encapsulation efficiency was calculated.The t-test was performed using SPSS 20.0 software,the differences between these two methods were compared. Result: In the pH 6-9,the oil-water partition coefficient of dichroa alkali hydrochloride increased with increasing of pH,which was between 0.016 and 1.44;the recovery rate of dichroa alkali hydrochloride after sterilization was 37.16%-57.91%.Between the dextran gel microcolumn centrifugation and the cation exchange resin microcolumn centrifugation,there was no significant difference in the entrapment efficiency of the liposomes. Conclusion: Dichroa alkali hydrochloride is suitable for preparation of liposomes.However,its stability is not ideal,so the experimental temperature should be strictly controlled in the preparation process.Dextran gel microcolumn centrifugation and cation exchange resin microcolumn centrifugation can be used to determine the entrapment efficiency of dichroa alkali hydrochloride liposomes,and the cation exchange resin microcolumn centrifugation is suggested after comparison.
