Interventional Effect and Mechanism of Extract of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on Vascular Endothelial Cellular Senescence Induced by High Glucose and High Fat
10.13422/j.cnki.syfjx.20190125
- VernacularTitle: 人参-三七-川芎提取物对高糖高脂诱导血管内皮细胞衰老的影响
- Author:
Xue WANG
1
;
Cheng-kui XIU
1
;
Jing YANG
1
;
Jing-yi FANG
2
;
Yan LEI
1
Author Information
1. Medical Experiment Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Chinese Medicine Research Institute of Guangdong Pharmaceutical University, Guangdong Research Center for Integrative Medicine in Metabolic Diseases, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
replenishing Qi and activating blood circulation medicine;
extract of Ginseng Radix et Rhizoma,Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma;
vascular endothelium;
cell senescence
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):124-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mechanism of extract of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma in delaying the senescence of vascular endothelial cells induced by high glucose and high fat. Method: The 40 mmol·L-1 glucose and 100 μmol·L-1 palmitate were used to induce endothelial cell senescence. The experiment was divided into control group, model group and low, medium and high-dose traditional Chinese medicine groups (50,100,200 mg·L-1). The intervention lasted for 48 h. Cell proliferation was detected by cell counting kit-8(CCK-8); cell senescence was detected by senescence β-galactosidase staining; p16 and p21 protein expression levels were detected by Western blot; p-H2A. X(Ser139) expression, mitochondria ROS(mtROS) production and changes in mitochondrial membrane potential(MMP) were detected by immunofluorescence. Result: Compared with the control group, in model group, the cell proliferation ability and the number of SA-β-gal blue-stained cells decreased(P<0.01), while the mtROS production and the expressions of p16, p21 and p-H2A. X(Ser139) increased(P<0.05,P<0.01). Compared with the model group, extract of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma can increase cell proliferation and MMP levels(P<0.01), and reduce the number of SA-β-gal blue-stained cells, the mtROS production, and expression levels of p16, p21 and p-H2A. X(Ser139)(P<0.05,P<0.01). Conclusion: The above results suggest that extract of Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma delay of endothelial cellular senescence induced by high glucose and high fat, and its mechanism may be related to increasing mitochondrial membrane potential and reducing DNA damage accumulation caused by ROS production.
