Protective Effect of Renshen Sinitang and Its Active Ingredients on Myocardial Cell Injury Induced by Pentobarbital Sodium
10.13422/j.cnki.syfjx.20190124
- VernacularTitle: 人参四逆汤及其有效成分对戊巴比妥钠所致心肌细胞损伤模型的保护作用
- Author:
Shuo LI
1
;
Ping SU
2
;
Guang-ping ZHANG
2
;
Teng-fei CHEN
2
;
Li-na MA
2
;
Han LI
2
;
Hong-ping HOU
2
;
Zhong-xiu ZHANG
2
;
Yi-fei YANG
2
;
Yun-hang GAO
2
;
Ling SONG
2
;
Zu-guang YE
2
Author Information
1. School of Life Science, Liaoning Normal University, Dalian 116000, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100075, China
- Publication Type:Research Article
- Keywords:
heart failure;
Renshen Sinitang;
malondialdehyde (MDA);
Na+-K+-adenosine triphosphate (ATP) ase;
Ca2+-ATPse;
B-cell lymphoma-2 associated X protein (Bax)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):90-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the protective effect and mechanisms of Renshen Sinitang and its active ingredients on cardiomyocyte injury induced by pentobarbital sodium. Method: H9C2 cells were sub-cultured with ginsenoside Rb2 0.01, 0.1, 1 μmol ·L-1, Re 0.01, 0.1, 1 μmol·L-1, isoliquiritigenin 20, 40, 80 μmol·L-1, glycyrrhetinic acid 10, 20, 40 μmol·L-1, Renshen Sinitang, 10, 100, 400 mg·L-1, for 4 h. After treatment with 0.1% of sodium pentobarbital for 30 min, cell viability, lactate dehydrogenase (LDH), lipid peroxide malondialdehyde (MDA), Na+-K+-adenosine triphosphate(ATP) ase, Ca2+-ATPase activity, and real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) were used to detect the expressions of peroxisome proliferative activated receptor-1α (PGC-1α), B-cell lymphoma-2 associated X protein(Bax) and cysteine aspartate-specific protease-3(Caspase-3) mRNA. Result: Renshen Sinitang and its active ingredients have a protective effect on heart failure cell model. Compared with the normal group, the cell survival rate of the model group decreased significantly, while the LDH and MDA contents increased significantly, and the Na+-K+-ATPase activity increased. Ca2+-ATPase activity was significantly decreased, PGC-1α mRNA expression was down-regulated, Bax and Caspase-3 mRNA expressions indicates the modeling(P<0.01). Compared with the model group, each administration group showed a significantly increased cell viability, decreased LDH, MDA content, inhibited Na+-K+-ATPase activity, increased Ca2+-ATPase activity, up-regulated PGC-1α mRNA expression, and inhibited Bax and Caspase-3 mRNA expression (P<0.05, P<0.01). Conclusion: Renshen Sinitang and its active ingredients have a significant protective effect on heart failure cell model, and its mechanisms of action are related to anti-oxidation, improvement of mitochondrial energy metabolism and inhibition of mitochondrial apoptosis pathway.
