Effect of Kangxianling Decoction on TGF-β1/Smad Signal Pathway in Renal Fibrosis Rats
10.13422/j.cnki.syfjx.20182036
- VernacularTitle: 抗纤灵方对肾纤维化大鼠TGF-β1/Smad信号通路表达的影响
- Author:
Jing JI
1
;
Li-qun HE
1
Author Information
1. Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 200123, China
- Publication Type:Research Article
- Keywords:
Kangxianling decoction;
renal fibrosis;
5/6 nephrectomy;
transforming growth factor-β1(TGF-β1);
Smad2;
Smad3;
Smad7
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):69-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the therapeutic effect of Kangxianling Decoction on renal fibrosis induced by 5/6 nephrectomization in rats, and discuss its action mechanism. Method: Totally 50 SD rats were randomly divided into normal control group (n=10), sham-operation group (n=10), and 5/6 nephrectomized renal fibrosis model group (n=30). After successful modeling, rats were randomly divided into the model group, Kangxianling group, and Losartan Potassium group. Rats in the Losartan Potassium group were administered with Losartan Potassium by gastrogavage; rats in the Kangxianling group were administered with Kangxianling by gastrogavage. Equal volume of distilled water was administered to rats in the other groups. Rats were sacrificed after 8 weeks of consecutive medication, and then serum creatinine (SCr), urea nitrogen (BUN), 24 hour urine protein (24 h-Pro) were measured in each group. Hematoxylin-eosin (HE) staining was used to observe the renal pathological changes and the score of Kidney damage was made. Masson staining was used to observe the degree of renal fibrosis. Western blot and real-time fluorescent quantitative polymerase chain reaction(Real-time PCR) were used to detect the protein and mRNA expression levels of transforming growth factor (TGF-β1), Smad2, Smad3, and Smad7 in kidneys. Result: As compared with the normal control and sham-operation group, the renal tissue fibrosis, score of kidney damage, SCr, BUN and 24 h-Pro levels were higher in model group (P<0.01), with increased protein and mRNA expression levels of TGF-β1, Smad2, and Smad3 (P<0.01), and significantly reduced protein and mRNA expression levels of Smad7 in the model group (P<0.01). As compared with the model group, the renal tissue fibrosis, the score of kidney damage, SCr, BUN, 24 h-Pro level were significantly decreased (P<0.01), protein and mRNA expression levels of TGF-β1, Smad2, Smad3 were reduced (P<0.05), while protein and mRNA expression levels of Smad7 were elevated in the Losartan Potassium group and Kangxianling group (P<0.01), but lower than those of normal control group and sham-operation group. Conclusion: Kangxianling decoction could alleviate renal fibrosis by inhibiting TGF-β1/Smad signal pathway.
