Effect of Kangxianling Decoction on Renal Function and Kidney ECM Expression in Rats with Renal Fibrosis
10.13422/j.cnki.syfjx.20190123
- VernacularTitle: 抗纤灵方对肾纤维化大鼠肾功能及肾组织ECM表达的影响
- Author:
Jing JI
1
;
Li-qun HE
1
Author Information
1. Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 200123, China
- Publication Type:Research Article
- Keywords:
Kangxianling decoction;
extracellular matrix (ECM);
renal fibrosis;
collagen Ⅰ;
collagen Ⅲ
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):63-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Kangxianling decoction on renal function and expression of extracellular matrix(ECM) in renal tissue of rats with renal fibrosis induced by 5/6 nephrectomy. Method: Totally 50 SD rats were randomly divided into control group (n=7), sham-operation group (n=7), operation group (n=36). The 5/6 nephrectomized renal fibrosis model was established in operation group. After successful modeling, rats were randomly divided into model group, Kangxianling group and losartan potassium group. The losartan potassium group and the Kangxianling group were given losartan potassium (33.3 g·kg-1) and Kangxianling decoction (21 g·kg-1) every day. The control group, sham-operation group and model group were all treated with equal volume of normal saline. Rats were put to death after 24 weeks of consecutive medication, and renal function, 24 h urine volume and 24 h-Pro quantitation of each group were measured. The expressions of collagen Ⅰ(ColⅠ) and ColⅢ in renal tissues were determined by immunohistochemistry and Western blot. Result: Compared with the normal group and sham-operation group, serum creatinine(SCr), blood urea nitrogen(BUN), 24 h urine volume, 24 h-Pro quantitation, renal tissue ColⅠ and ColⅢ expression levels were significantly increased in the model group (P<0.01). Compared with the model group, the levels of SCr, BUN, 24 h-Pro quantitation, protein expression levels of renal tissue ColⅠ and ColⅢ were significantly decreased in the Kangxianling group and the losartan potassium group (P<0.05,P<0.01). Conclusion: Kangxianling decoction can down-regulate the expressions of ColⅠ and ColⅢ in kidney tissue, there by inhibiting the accumulation of ECM, and exerting the effect in delaying renal fibrosis and protecting renal function.
