Effect of Kangxianling Decoction on Renal Fibrosis and ACE-AngⅡ-AT1R Axis in 5/6 Nephrectomized Rats
10.13422/j.cnki.syfjx.20190122
- VernacularTitle: 抗纤灵方对5/6肾切除大鼠肾纤维化及ACE-AngⅡ-AT1R轴的影响
- Author:
Jing JI
1
;
Li-qun HE
1
Author Information
1. Shanghai University of Traditional Chinese Medicine(TCM), Shanghai 200123, China
- Publication Type:Research Article
- Keywords:
5/6 nephrectomy;
renal fibrosis;
Kangxianling decoction;
angiotensin converting enzyme-angiotensin Ⅱ-angiotensin Ⅱ 1 receptor (ACE-AngⅡ-AT1R)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the therapeutic effect of Kangxianling decoction on renal fibrosis induced by 5/6 nephrectomy, and angiotensin converting enzyme-angiotensin Ⅱ-angiotensin Ⅱ 1 receptor (ACE-AngⅡ-AT1R) axis. Method: Totally 50 SD rats were randomly divided into the following groups:control group (n=10), sham-operation group (n=10), 5/6 nephrectomized renal fibrosis model group (n=30). After two weeks, the rats in operation group were divided into the model group, Kangxianling group, and losartan potassium group, n=10 in each group. Rats in losartan potassium group were administered with losartan potassium by gastrogavage, and rats in Kangxianling group were administered with Kangxianling by gastrogavage. Equal volume of saline was administered to rats in the other groups. The rats were put to death after 16 weeks of consecutive medication, and serum creatinine(SCr), blood urea nitrogen(BUN), 24 h urine protein(24 h-Pro) were measured in each group. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of kidney tissues, and the degree of renal fibrosis was observed by Masson staining. The expressions of ACE1 and AT1R were detected by immunohistochemistry. The protein expression levels of ACE1, AngⅡ and AT1R were determined by Western blot. Result: Compared with control and sham-operation groups, SCr, BUN and 24 h-Pro in model group were significantly increased (P<0.01). Compared with model group, SCr, BUN and 24 h-Pro levels in the Kangxianling group and the losartan potassium group were significantly lower, with statistically significant differences (P<0.05,P<0.01). HE and Masson staining showed that the degree of renal fibrosis was reduced in Kangxianling group and losartan potassium group compared with model group. Western blot showed that ACE1, AngⅡ and AT1R in Kangxianling group, and the losartan potassium were significantly lower than the model group, with statistically significant differences (P<0.01). Immunohistochemistry results showed that the levels of ACE1 and AT1R showed the same changes as Western blot. Conclusion: Kangxianling decoction can delay the progress of renal fibrosis in 5/6 nephrectomized rats, which is closely related to the inhibition of ACE-AngⅡ-AT1R axis activation.
