Antioxidation Activities in Vitro of Vicenin Ⅱ Isolated from Dendrobii Officinalis Caulis and Effect on HepG2 Cells
10.13422/j.cnki.syfjx.20182210
- VernacularTitle: 铁皮石斛共性黄酮类成分新西兰牡荆苷Ⅱ的体外抗氧化与诱导HepG2细胞凋亡的作用
- Author:
Ying-yi LUO
1
;
Yun-rong LI
2
;
Zhou-xi LEI
1
;
Yuan YUAN
3
;
Yue-chun HUANG
4
;
Gang WEI
1
Author Information
1. Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Guangxi International Zhuang Medical Hospital, Nanning 530021, China
3. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
4. The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
- Publication Type:Research Article
- Keywords:
Dendrobii Officinalis Caulis;
flavonoid glycoside;
vicenin Ⅱ;
antioxidant;
HepG2 cell;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(1):43-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the antioxidation activities in vitro of a comment flavonoid component named vicenin Ⅱ(Apigenin 6,8-di-C-glucoside) in Dendrobii Officinalis Caulis from different origin places and investigate its effects on apoptosis of HepG2 cells. Method: The antioxidation activities in vitro of vicenin Ⅱ (0.005-1 g·L-1) were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), salicylic acid and 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS) and copper ion reduction assays. Methye thiazolye telrazlium(MTT) assay was used to test the inhibitory effect of vicenin Ⅱ(12.5~100 μmol·L-1) on proliferation of 6 tumour cells in vitro. In subsequent apoptosis experiment, the concentration of vicenin Ⅱ was 75 μmol·L-1. The morphological changes of HepG2 cells were evaluated by Hoechst 33258 under fluorescence microscope; and the cell apoptosis rate was detected by flow cytometry with AnnexinV/PI apoptosis assay kit. The mRNA expressions of mitogen activated protein kinase (MAPK) pathway related apoptotic genes were detected by Real-time PCR assay. Result: The 1 g·L-1 vicenin Ⅱ showed 48.82% and 22.01% for DPPH scavenging rate and Cu2+ reduction rate respectively(P<0.01). 0.5 g·L-1 vicenin Ⅱ showed 86.88% for ABTS scavenging rate(P<0.01). vicenin Ⅱ could significantly inhibit the proliferation and increase the apoptosis rate on HepG2 cells; after treatment for 48 h with 75 μmol·L-1 Vicenin Ⅱ, the cells survival rate was 45.69%(P<0.01) and apoptotic rate was 14.57%(P<0.01). Meanwhile, the mRNA expression levels of B-cell lymphoma-2 (Bcl-2) related X protein (Bax)/Bcl-2, Caspase-8, p38, extracellular signal-regulated kinases (ERK), c-Jun N-terminal kinase (JNK), and nuclear transcription factor (NF)-κB were increased(P<0.01). Conclusion: The general flavone glycosides component vicenin Ⅱ of Dendrobii Officinalis Caulis from different origins has a certain antioxidation effect and significant inhibitory effect on proliferation, and could induce apoptosis on HepG2 cells probably by regulating the expression of related genes in MAPK pathway and Bax/Bcl-2.
