Effects of RFX1 over-expression on proliferation and invasion of glioma cells
10.3872/j.issn.1007-385x.2018.10.008
- VernacularTitle:过表达RFX1对胶质瘤细胞增殖和侵袭的影响
- Author:
CHENG Kai
1
;
ZHANG Yaping
2
;
CAO Li
2
;
ZHANG Xiaoyan
3
Author Information
1. Department of Biochemistry
2. Science and Technology Center
3. Department of Pathogenic Biology, Fenyang College of Shanxi Medical University
- Publication Type:Journal Article
- Keywords:
malignant glioma;
F98 cell line;
regulatory factor X1（RFX1）;
proliferation；invasion；apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(10):1021-1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To validate the effect and the possible mechanism of human regulatory factor X1 (RFX1) over-expression on the proliferation and invasion of glioma F98 cells. Methods: RFX1-overexpressed F98 cells (F98-RFX1 group) were constructed by lentivirus transfection, a control group (F98-Vector group) and normal group (F98 group) were established. The effect of RFX1 over-expression on F98 cell proliferation was observed with counting method, cell apoptosis was determined by AnnexinV-PI staining, and the cell invasion was observed with Transwell method. Results: F98 cell line over-expressing RFX1 was successfully established. The proliferation capacity of F98-RFX1 group was significantly lower than that of F98 group (48 h: [12.08±2.17]×104/ml vs [23.67±4.51]×104/ ml, P<0.05] and F98-Vector group (96 h: [8.17±0.31]×104/ml vs [18.58±1.18]×104/ml, P<0.05); The apoptosis level of cells in F98RFX1 group was significantly increased ([21.89±2.33]% vs [3.38±1.39]%, [10.42±1.83]%, P<0.05]; The invasiveness of cells in F98RFX1 group was significantly reduced ([33.3±7.99] vs [56.5±13.9], [60.6±11.8], P<0.01). Conclusion: RFX1 can regulate the expression of genes related with proliferation and invasion, thereby inhibiting the proliferation and invasion ability of glioma cells and promote cell apoptosis.
- Full text:20181008.pdf
