Comparative study on the inhibitory effect of dual specific oncolytic adenovirus and doxorubicin on breast cancer cells
10.3872/j.issn.1007-385x.2018.11.013
- VernacularTitle:双特异性溶瘤腺病毒和多柔比星对乳腺癌细胞增殖抑制作用的对比分析
- Author:
LI Shanzhi1
1
,
2
;
CHEN Shuang2
3
,
4
;
ZHAO Jin2
5
;
LI Yiquan2
6
;
ZHU Yilong2
3
,
4
;
LI Wenjie2
5
;
YIN Xunzhe2
6
;
CUI Yingli
5
;
WANG Jing2
5
;
LIU Xing2
5
;
LI Xiao2
5
;
JIN Ningyi2
5
Author Information
1. 1. College ofAnimal Science and Technology, JilinAgricultural University, Changchun 130118, Jilin, China
2. 2.Key Laboratory of Molecular Virology and Immunology, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, Jilin, China
3. 2.Key Laboratory of Molecular Virology and Immunology, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, Jilin, China
4. 3. College of Examination, Jilin Medical College,Jilin 132013, Jilin, China
5. Key Laboratory of Molecular Virology and Immunology, Institute of Military Veterinary, Academy of Military Medical Sciences
6. .Key Laboratory of Molecular Virology and Immunology, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, Jilin, China
- Publication Type:Journal Article
- Keywords:
breast cancer cells;
oncolytic adenovirus;
doxorubicin;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
2018;25(11):1159-1165
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the difference in the proliferation inhibition of doxorubicin and dual specific oncolytic adenoviruses (Ad-VT, Ad-T, Ad-VP3 and d-Mock) on breast cancer cells and normal mammary cells. Methods: The proliferation inhibition rates of doxorubicin and recombinant adenovirus(Ad-VT, Ad-T, Ad-VP3and Mock) on breast cancer cells were detected through WST-1 experiment, and the effects of two drugs on the inhibitory rates of normal mammary epithelial cells were also detected. Moreover, the apoptosis rates of doxorubicin and oncolytic adenoviruses on breast cancer cells and normal mammary epithelial cells were evaluated by Annexin V flow cytometry, Hoechst and JC-1 staining, and the difference in the apoptosis rates were also compared. Results: All the recombinant adenovirus could effectively suppress the proliferation of breast cancer cells (P<0.05 or P<0.01), the inhibition effects followed the order ofAd-VT>Ad-T>Ad-VP3>Ad-MOCK, and the inhibition effect was positively correlated with time. Doxorubicin could also effectively suppress the proliferation of breast cancer cells (P<0.05 or P<0.01), and the inhibition effect was markedly enhanced with the increases in does and time. However, doxorubicin also showed strong inhibition effect on the normal mammary epithelial cells, and the inhibition rate achieved 80% under 72 h and 5 ug/ml doxorubicin, while that of oncolytic adenovirus Ad-VT on MCF-10A was 20% at 72 h. The apoptosis effects of oncolytic adenoviruses-induced breast cancer cellwere increased with time, and the apoptosis rate efficiency followed the order of Ad-VT>Ad-T>Ad-VP3>Ad-MOCK, but they displayed low ability to induce normal mammary cell apoptosis. The apoptosis effects of doxorubicin-induced breast cancer cell were similar to that of the normal mammary epithelial cell (P <0.05 or P<0.01), which followed the dose of 0.05<0.5<5 μg/ml. Conclusion: Dual specific oncolytic adenoviruses can effectively suppress the proliferation of breast cancer cells, but they have low inhibition on normal mammary cells, which have displayed superior safety and provide a new method for the biotherapy of tumor.
- Full text:20181113.pdf
