Inhibitory effect of recombinant oncolytic adenovirus on luciferase-labeled and non-labeled human lung cancerA549 cells
10.3872/j.issn.1007-385x.2018.12.009
- VernacularTitle:重组溶瘤腺病毒对荧光素酶标记和非标记人肺癌 A549 细胞抑制作用 比较
- Author:
MA Yizhen
1
,
2
;
FAN Yuanyuan
1
,
2
;
NIE Xi
3
,
4
;
SUN Lili
5
;
ZHU Yilong
1
,
2
;
LI Yiquan
6
;
LI Wenjie
6
;
YIN Xunzhe
1
,
2
;
LI Shanzhi
6
;
ZHAO Jin
6
;
LI Xiao
6
;
GUO Yan
7
;
JIN Ningyi
6
Author Information
1. (1. Changchun University of Traditional Chinese Medicine, Changchun 130117, Jilin, China
2. 2. Laboratory of Molecular Virology and Immunology, Institute of Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun 130122, Jilin, China
3. Unit 65316, People&rsquo
4. s Liberation Army, Changchun 130000, Jilin, China
5. 4. Tumor Hospital of Jilin Province, Changchun 130021, Jilin, China
6. 2. Laboratory of Molecular Virology and Immunology, Institute of Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun 130122, Jilin, China
7. 1. Changchun University of Traditional Chinese Medicine, Changchun 130117, Jilin, China
- Publication Type:Journal Article
- Keywords:
oncolytic adenovirus;
luciferase;
A549-luc cell;
inhibitory effect
- From:
Chinese Journal of Cancer Biotherapy
2018;25(12):1264-1269
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect of recombinant oncolytic adenovirusAd-Apoptin-hTERTp-E1A(ATV) on luciferase-labeled human lung cancer cells (A549-luc) and human lung cancerA549 cells, and to compare the differences in the inhibitory effect on two cell lines. Methods:ATV was used to infectA549-luc cells andA549 cells respectively. WST-1 and crystal violet staining were used to determine the difference in the inhibitory effect of ATV. Hoechst and Annexin V-FITC/PI staining were used to verify the inhibition mode ofATV. Results: WST-1 and crystal violet staining showed thatATV had significant inhibitory effect on bothA549-luc and A549 cells ( P <0.05). ATV showed significant inhibitory effect on both cells at 24, 48 and 72 h ( P <0.05 or P <0.01), and reached the peak at 72 h; ATV at concentrations of 1, 10 and 100 MOI all showed inhibitory effect on both cells, and reached the peak at 100 MOI. Hoechst staining showed that A549-luc cells and A549 cells infected with ATV showed typical nuclear fragmentation and marginal set. The results of Annexin V-FITC/PI Flow cytometry showed that ATV infection resulted in apoptosis of A549-luc and A549 cells, which was in a time-dependent manner and reached the peak at 72 h( P <0.05 or P <0.01). Conclusion: Insertion of luciferase didn’t significantly change the inhibitory effect and inhibitory mode ofATV onA549-luc cells.ATV exerted its in vitro inhibitory effect onA549-luc and A549 cells by inducing cell apoptosis.
- Full text:20181209.pdf