Effect of miR-141-3p targeting TGF-β2 on malignant biological behaviors of human prostatic cancer C4-2B cells
10.3872/j.issn.1007-385x.2018.12.005
- VernacularTitle:miR-141-3p靶向TGF-β2 对人前列腺癌 C4-2B 细胞恶性生物学行为的 影响
- Author:
WANG Yu
1
;
QIU Mingxing
2
,
3
;
XIONG Guobing
2
,
3
Author Information
1. Department of Urology Surgery, the Affiliated Hospital of Southwestern Medical University
2. . Department of Urology Surgery, Sichuan Provincial People'
3. s Hospital, Sichuan Academy of Medical Sciences
- Publication Type:Journal Article
- Keywords:
prostate cancer;
C4-2B cell；miR-141-3p;
transforming growth factor-β2 (TGF-β2);
proliferation;
apoptosis;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2018;25(12):1237-1243
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the relationship between miR-141-3p and transforming growth factorβ2 (TGF-β2), and its effects on the malignant biological behaviors of human prostate cancer cell line C4-2B. Methods:After the transfection of miR-141-3p mimic, the mRNAexpression of miR-141-3p and TGF-β2 in C4-2B cells was detected by qRT-PCR. Bioinformatics method validated the relationship between miR-141-3p and TGF-β2. miR-141-3p mimic alone or with TGF-β2 over-expression vector was transfected into C42B cells, and then Western blotting was used to detect the expression of TGF-β2 protein in C4-2B cells, Hochest33258 staining was used to detect cell apoptosis, and Transwell assay was used to detect the invasion ability of cells in each group. Results:After the transfection of C4-2B cells with miR-141-3p mimic, the level of miR-141-3p increased significantly, and the level of TGF-β2 mRNA decreased significantly (all P<0.01). The activity of luciferase was significantly reduced after the co-transfection with miR-141-3p mimic and wild type report plasmid (P<0.01); However, the activity of luciferase was not obviously changed after co-transfection with miR141-3p mimic and mutant type report plasmid (P>0.05).After co-transfection with miR-141-3p mimic and pc-TGF-β2, the proliferation of C4-2B cells decreased significantly, the number of apoptotic cells increased significantly, and the cell invasion ability decreased significantly (all P<0.01). Conclusion: miR-141-3p inhibits the proliferation and invasion of human prostate cancer C4-2B cells and induces cell apoptosis by targeting TGF-β2.
- Full text:20181205.pdf
