Expressions of hypoxia inducible factor-1α and autophagy related protein Beclin-1 in colon cancer and their clinical significances
10.3760/cma.j.issn.1006-9801.2019.11.007
- VernacularTitle: 缺氧诱导因子1α和自噬相关蛋白Beclin-1在结肠癌中的表达及其临床意义
- Author:
Zhongguo LI
1
;
Yaoqiang WU
;
Hongwei XUE
;
Fuxiang YU
Author Information
1. Department of General Surgery 1, Dandong First Hospital of Liaoning Province, Dandong 118000, China
- Publication Type:Journal Article
- Keywords:
Colon neoplasms;
Hypoxia-inducible factor 1, alpha subunit;
Gene, Beclin-1;
Autophagy
- From:
Cancer Research and Clinic
2019;31(11):751-755
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions of hypoxia inducible factor-1α (HIF-1α) and autophagy related protein Beclin-1 in colon cancer and their clinical significances.
Methods:From January 2017 to December 2018, 120 patients with colon cancer who were admitted to Dandong First Hospital were selected. The expressions of HIF-1α and Beclin-1 in colon cancer and corresponding adjacent tissues were detected by immunohistochemical SABC method. The relationship of the expressions of HIF-1α and Beclin-1 with the clinicopathological features of colon cancer patients was also discussed. The expressions of HIF-1α and Beclin-1 in 20 pairs of fresh colon cancer and paracancerous tissues were detected by Western blot.
Results:The results of immunohistochemistry showed that the positive expression rates of HIF-1α and Beclin-1 proteins in colon cancer tissues were significantly higher than those in paracancerous tissues [75.0% (90/120) vs. 21.7% (26/120), 60.8% (73/120) vs. 12.5% (15/120)], and the differences were statistically significant (χ 2 values were 68.343 and 60.359, both P < 0.01). The results of Western blot showed that the expressions of HIF-1α and Beclin-1 proteins in 20 pairs of fresh colon cancer tissues were significantly higher than those in paracancerous tissues (1.98±0.66 vs. 0.76±0.55, 1.50±0.40 vs. 0.46±0.35), and the differences were statistically significant (t values were 6.912 and 8.315, both P < 0.01). The expressions of HIF-1α and Beclin-1 in colon cancer tissues were related to TNM stage, degree of differentiation, depth of infiltration, lymph node metastasis and nerve invasion (HIF-1α: χ 2 values were 9.074, 15.215, 10.101, 11.610 and 9.979; Beclin-1: χ 2 values were 11.285, 4.858, 24.436, 9.354 and 4.632; all P < 0.05), but not with age, sex, tumor location, tumor diameter and vascular tumor thrombus (all P > 0.05). There was a positive correlation between the expressions of HIF-1α and Beclin-1 proteins in colon cancer (r = 0.483, P < 0.01).
Conclusion:The expressions of HIF-1α and Beclin-1 proteins in colon cancer tissues are significantly increased, and the interaction between them is involved in the occurrence and development of colon cancer.
