Study on a high-throughput method with LC-MS/MS technique for detecting aflatoxins in ginger
10.3760/cma.j.issn.1673-4246.2019.11.018
- VernacularTitle: 生姜中黄曲霉毒素液质联用高通量检测研究
- Author:
Ying ZHANG
1
;
Wenchao LIU
1
;
Yanli YANG
1
;
Zhiqiang SHU
2
;
Lang LI
1
Author Information
1. Sun Simiao School of Medicine, Tongchuan Vocational and Technical College, Tongchuan 727031, China
2. Shaanxi origin Agricultural Technology Co., Ltd., Tongchuan 727031, China
- Publication Type:Journal Article
- Keywords:
Zingiberis rhizoma recens;
Tandem mass spectrometry;
Aflatoxins;
High-throughput determination
- From:
International Journal of Traditional Chinese Medicine
2019;41(11):1236-1240
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the method of simultaneous determination of four aflatoxins (B1, B2, G1 and G2) of ginger by the ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method and high-throughput method.
Methods:The aflatoxins were extracted from ginger by methanol-water (80:20, V/V) solution, concentrated and dried with nitrogen. The aflatoxins were detected by UPLC-MS/MS by using Waters Acquity UPLC BEH C18 chromatographic column. The mobile phase was 0.1% formic acid water (A phase) -0.1% formic acid methanol (B phase), gradient elution, flow rate 0.35 ml/min, mass spectrometry was electrospray ion source, positive ion scanning mode, multi reaction ion monitoring were using.
Results:Quantification of four aflatoxins by matrix matching standard curve. The linear was good in the range of 0.125-20.000 ng/ml, and the correlation coefficients were all greater than 0.999 0. The ginger sample detection was 0.125-0.300 μg/kg and 0.125-1.000 μg/kg, respectively. The average recoveries were 81.7%-96.0%, and the relative standard deviation (RSD) was lower than 7.53%.
Conclusions:This method is simple, rapid, sensitive and low limit of detection, which can meet the requirements for the detection of trace aflatoxins residues in ginger.
