Effect of different proportions of piper chinaroot and rhubarb on the dissolution rate of five effective components in rhubarb
10.3760/cma.j.issn.1673-4246.2019.11.017
- VernacularTitle: 荜茇-大黄不同比例配伍对大黄中5种有效成分溶出率的影响
- Author:
Hong CHEN
1
;
Yi HAN
;
Mengxi QIAN
;
Xiaohui SHI
;
Yujuan MAO
Author Information
1. Department of Pharmacy of Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang 215600, China
- Publication Type:Journal Article
- Keywords:
Piperis longi fructus;
Rhei radix et rhizoma;
Compatibility (TCD);
Aloeemodin;
Emodin;
Chrysophanol;
Rhein;
Physcion;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2019;41(11):1231-1235
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of different proportions of piper chinaroot and rheum palmatum on the dissolution rate of five effective components (aloe emodin, emodin acid, emodin, emodin, emodin methyl ether).
Methods:The high performance liquid chromatography (HPLC) method was used to analyz the contents of five effective components of rheum palmatum in the extracts of different combination of piper chinaroot and rheum palmatum. The tests were carried out by Thermo C18 (250 mm×4.6 mm, 5 μm) by gradient elution with methanol and 0.1% phosphoric acid water solution as mobile phase at a flow rate of 1ml/min, the column temperature was 30 ℃ and the detection wavelength was 254 nm.
Results:The linear ranges of aloe emodin, emodin acid, emodin, emodin, emodin methyl ether were 0.018 5-0.741 8, 0.017 9-0.717 8, 0.015 9-0.635 5, 0.054 2-2.167 2, 0.016 2-0.646 4 μg, respectively. The average recoveries of aloe emodin, emodin acid, emodin, emodin, emodin methyl ether were 94.35%, 95.50%, 100.61%, 96.27%, 97.39%, and the RSDs were 1.81%, 1.99%, 2.84%, 2.71%, and 1.86%, respectively. Compared with rheum palmatumby single extract, the content of aloe emodin and emodin were increased by 5 proportions (3:1, 2:1, 1:1, 1:2, 1:3), while the content of emodin and emodin methyl ether were decreased.
Conclusions:The optimal compatibility proportion of piper chinaroot and rheum palmatum is 1:1.
