Rhizoma Pleionis inhibits of breast cancer epithelial-mesenchymal transition by VEGF-A related angiogenesis
10.3760/cma.j.issn.1008-1372.2019.11.014
- VernacularTitle: 山慈菇介导VEGF-A血管生成抑制乳腺癌细胞上皮-间质转化的实验研究
- Author:
Xuelian LI
1
;
Zhi LI
1
;
Qian HUANG
2
;
Ruixiao LI
3
Author Information
1. Department of Surgical, Xi′an Chinese Medicine Hospital, Xi′an 710002, China
2. Department of Pharmacy, Xi′an Chinese Medicine Hospital, Xi′an 710002, China
3. Department of Urology, the Second Affiliated Hospital of Air Force Military Medical University, Xi′an 710038, China
- Publication Type:Journal Article
- Keywords:
CREMASTRA VARIABILIS;
Vascular endothelial growth factor A;
Breast neoplasms;
Tumor cells, cultured;
Epithelial-mesenchymal transition
- From:
Journal of Chinese Physician
2019;21(11):1658-1662
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the Rhizoma Pleionis mediating vascular endothelial growth factor A (VEGF-A)-related angiogenesis in breast cancer cells to inhibit epithelial-mesenchymal transition (EMT) in breast cancer.
Methods:Firstly, the proliferation activity of MDA-MB-231 and 4T1 of breast cancer cells was detected by methyl thiazolyl tetrazolium (MTT) method at different concentrations (0.2 g/ml, 0.4 g/ml, 0.6 g/ml, 0.8 g/ml, 1.0 g/ml); secondly, the specific concentration of Rhizoma Pleionis was selected and tested by Transwell test for its effect on breast cancer cell metastasis and invasion. The formation of human umbilical vein endothelial cells (HUVEC) capillary wall was measured to detect the effect of Rhizoma Pleionis on cell angiogenesis. The EMT-related protein and angiogenesis-related molecules were detected by Western blot.
Results:MTT results showed that Rhizoma Pleionis of 0.8 g/ml and 1.0 g/ml can inhibit the MDA-MB-231 and 4T1 cells proliferation in a dose-dependent manner, with statistically significant difference (P<0.05). Rhizoma Pleionis can inhibit the invasion of breast cancer cells and inhibit the formation of blood vessel walls in HUVECs. Western blot confirmed that the expression level of EMT-related proteins: E-cadherin was significantly increased and the expression level of N-cadherin, Vimentin and Snail was significantly decreased after treatment with Rhizoma Pleionis (P<0.05); and the level of the related molecule hypoxia inducible factor-1α (HIF-1α), VEGF-A and vascular endothelial growth factor receptor-2 (VEGFR-2) was significantly reduced (P<0.05).
Conclusions:Rhizoma Pleionis inhibits the epithelial-mesenchymal transition of breast cancer by influencing the angiogenesis of VEGF-A, and inhibits the invasion and metastasis of breast cancer.
