Resistance of osteosclerotin knockout mice to glucocorticoid induced bone microstructure degeneration
10.3760/cma.j.issn.1008-1372.2019.11.008
- VernacularTitle: 骨硬化素基因敲除小鼠抵抗糖皮质激素诱导的骨微结构退变的研究
- Author:
Yulin MA
1
;
Miaolan YUAN
1
;
Xinyun DUAN
1
;
Zhifeng SHENG
2
Author Information
1. Department of Endocrinology, the Affiliated Xiaolan Hospital, Southem Medical University, Zhongshan 528415, China
2. Institute of Metabolism and Endocrinology, the Second Xiangya Hospital, Central South University, Changsha 410011, China
- Publication Type:Journal Article
- Keywords:
Glucocorticoids;
Sclerotin;
Gene knockout techniques;
Bone density;
Mice
- From:
Journal of Chinese Physician
2019;21(11):1632-1635
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes in bone mineral density and microstructure parameters in sclerostin (SOST) gene knockout mice treated with glucocorticoid.
Methods:12 4-week-old SOST knockout mice were randomly divided into two groups (n=6): methylprednisolone intervention group [SOM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection], placebo group (SOS group, isovolumetric saline subcutaneous injection). 12 wild-type mice were randomly divided into two groups (n=6): wild-type placebo group (WTS group, isovolumetric saline subcutaneous injection), wild methylprednisolone intervention group [WTM group, methylprednisolone 3 mg/(kg·d), subcutaneous injection]. 12 weeks later, mice were sacrificed and one lumbar vertebra of each mouse was selected for micro-CT analysis.
Results:There was no difference in bone mineral density (BMD), trabecular volume fraction, trabecular number and trabecular thickness between SOM and SOS groups (P>0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in SOM and SOS groups were significantly higher than those in WTS and WTM groups (P<0.05). BMD, trabecular volume fraction, trabecular number and trabecular thickness in WTM group were significantly lower than those in WTS group (P<0.05).
Conclusions:Sclerotin gene knockout mice can resist glucocorticoid-induced bone loss and bone microarchitectural deterioeration. The treatment of osteoporosis with SOST/sclerotin as a target will be an effective method in the future.
