Investigation and research on ticks carrying spotted fever group rickettsia in the border area of Tumen River Basin
10.3760/cma.j.issn.0253-9624.2019.11.011
- VernacularTitle: 图们江流域边境地区蜱携带斑点热群立克次体调查研究
- Author:
Jixu LI
1
;
Roh JONG-YUL
2
;
Park WON-LL
2
;
Wen PIAO
3
;
Guangjun JIN
4
;
Zhonggao WU
5
;
Zhenhai SONG
5
;
Shenghuan QUAN
6
;
Guangming PIAO
7
;
Hao SONG
7
;
Cho SHIN-HYEONG
2
Author Information
1. Division of Vector Control, Yanbian Center for Disease Control and Prevention Yanji 133001, China
2. Division of Vectors and Parasitic Diseases, Korea Center for Disease Control and Prevention, Cheongju 363951, Korea
3. Hunchun Center for Disease Control and Prevention, Hunchun 133300, China
4. Division of Health surveillance, Hunchun Center for Disease Control and Prevention, Hunchun 133300, China
5. Division of Vector Control, Tumen Center for Disease Control and Prevention, Tumen 133100, China
6. Division of Infectious Disease Control, Helong Center for Disease Control and Prevention, Helong 133500, China
7. Division of Vector Control, Longjing Center for Disease Control and Prevention, Longjing 133400, China
- Publication Type:Journal Article
- Keywords:
Ticks;
Rickettsia infections;
Spotted fever group rickettsia;
Sequence analysis;
Polymerase chain reaction
- From:
Chinese Journal of Preventive Medicine
2019;53(11):1130-1135
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the situation and genotype distribution of spotted fever group rickettsia (SFGR) in the border area of Tumen River Basin in free ticks in Yanbian Korean Autonomous Prefecture (Yanbian Prefecture), Jilin Province.
Methods:From April to September, 2017, ticks were collected using flagging method from Hunchun, Tumen, Helong and Longjing cities in the Tumen River basin of Yanbian Prefecture. Outer membrane protein A (ompA) was detected by Polymerase Chain Reaction (PCR), then, the species were identified by gene sequencing and analyzed systematically. The positive rate of pools and MIR(minimum infection rate per 100 ticks,MIR) of SFGR were calculated, and the difference of positive rate of pools among ticks with different characteristics was compared by Chi-square test.
Results:A total of 3 079 ticks were collected and divided into 536 pools. The positive rate of pools of SFGR nucleic acid was 39.7% (213 pools). The MIR of SFGR was 6.9%.The positive rate of pools of SFGR in Dermacentor silvarum, Haemaphysalis concinna, Haemaphysalis japonica, Haemaphysalis longicornis and Ixodes persulcatus were 80.4% (41/51), 14.0% (25/179), 20.2% (18/89), 78.9% (101/128) and 25.9% (21/81), and the difference was statistically significant (P<0.001). There was statistical difference in the positive rate of pools of SFGR in developmental stages of ticks (P<0.001); the positive rate of pools of female adults, male adults, nymph and larvae were 36.4% (95/261), 34.2% (67/196), 56.3% (40/71) and 7/8, and the MIR was 7.9%, 7.7%, 4.9% and 3.5%. The five genotype was detected which was Candidatus Rickettsia longicornii, Rickettsia raoultii, Rickettsia heilongjiangensis, Candidatus Rickettsia tarasevichiae,Rickettsia monacensis and have 98%-100% homology with known gene sequences. Candidatus Rickettsia longicornii, Rickettsia raoultii, Rickettsia heilongjiangensis and Candidatus Rickettsia tarasevichiae showed close evolutionary relationship with known specie (have 98%-100% homology with known gene sequences); Rickettsia monacensis showed Far from evolutionary relationship with known species (have 98% homology with known gene sequences).
Conclusion:SFGR infection of ticks is common in the border areas of the Tumen River Basin. There was high diversity in SFGR species and tick species in the areas surveyed.
