Epidemiology investigation of carbapenems-resistant Klebsiella pneumoniae in burn care unit
10.3760/cma.j.issn.1009-2587.2019.11.006
- VernacularTitle: 烧伤监护室抗碳青霉烯类抗生素肺炎克雷伯菌的流行病学调查
- Author:
Yali GONG
1
;
Chunjiang LIU
2
;
Xiaoqiang LUO
1
;
Meixi LIU
1
;
Cheng ZHANG
1
;
Yunlong SHI
1
;
Yizhi PENG
1
Author Information
1. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, the First Affiliated Hospital of Army Medical University (the Third Military Medical University), Chongqing 400038, China
2. Clinical Laboratory of Shanxi Hospital, the Chinese People′s Armed Police Corps, Taiyuan 030006, China
- Publication Type:Journal Article
- Keywords:
Burns;
Klebsiella pneumoniae;
Drug resistance;
Cross infection;
Carbapenems
- From:
Chinese Journal of Burns
2019;35(11):798-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the resistance mechanism and gene type of carbapenems-resistant Klebsiella pneumoniae (CRKP) in burn care unit.
Methods:A total of 27 CRKP strains were primarily isolated from 22 patients [20 males, 2 females, aged (42±16) years] admitted to burn care unit of Institute of Burn Research of the First Affiliated Hospital of Army Medical University (the Third Military Medical University, hereinafter referred to as our department) from January to December 2017. After identification of bacteria, the months of detection and distribution of sample source were analyzed. Drug resistance tests of 15 antibiotics were conducted. Polymerase chain reaction was used to detect the drug resistant genes. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used to analyze the gene type of strains.
Results:(1) During the whole year of 2017, CRKP strains were mostly detected in August (8 strains), September (6 strains), and October (5 strains), with no CRKP in January, March, June, November, and December. Five strains from bed units were detected in August (2 strains), September (1 strain), and October (2 strains). (2) Twenty-seven CRKP strains were derived from blood samples (40.7%, 11/27), wound exudate samples (18.5%, 5/27), deep vein catheter samples (11.1%, 3/27), sputum samples (7.4%, 2/27), urine samples (3.7%, 1/27), and bed unit samples (18.5%, 5/27). (3) The 27 CRKP strains were detected with drug-resistance rates of 100.0% to 7 antibiotics including cefoperazone/sulbactam, piperacillin/tazobactam, cefazolin, ceftriaxone, cefepime, ertapenem, and compound sulfamethoxazole, no drug-resistance to tigecycline, with drug-resistance rates higher than 81.0% to the rest 7 antibiotics. (4) Detection rates for resistance gene blaCTX-M-10, blaSHV, blaTEM, blaCTX-M-14, blaACT, and blaKPC were all above 92.5%. (5) According to PFGE, the 27 CRKP strains had 6 types (A, A1, A2, B, C, and D). Strains of type A were mainly detected in February, May, and September, with detection rate of 37.0% (10/27). Strains of type C were mainly detected in July, August, and October, with detection rate of 48.1% (13/27). Strains of types A1, A2, B, and D were scatteredly detected, with detection rate of 3.7% (1/27) respectively. According to MLST, the 27 CRKP strains had 6 STs. ST11 was the most frequent type, accounting for 74.1% (20/27), which was detected in August to October. The detection rate of ST395, ST2230, ST215, ST260, and STnew ranged from 3.7%(1/27) to 7.4%(2/27), and the strains were scatteredly detected.
Conclusions:The main source of CRKP from burn care unit of our department was bloodstream. All the CRKP strains showed high drug-resistance rate and complicated resistance mechanism. There were small scale outbreaks caused by CRKP of type A, type C, and ST11, which should be paid more attention to in clinical treatment and infection control.
