CD34, VEGF and endothelin-1 expression in intrahepatic metastasis of hepatocellular carcinoma
10.3670.cma.j.issn.1007-631X.2019.11.006
- VernacularTitle: CD34、VEGF和内皮素－1在肝细胞癌肝内转移中的作用
- Author:
Jihong ZHANG
1
;
Chunlong HUANG
1
;
Shuai MAO
1
;
Rui CAO
1
Author Information
1. Department of Hepatobiliary Surgery, The Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen Universty. Guangzhou 510070, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Neoplasm metastasis;
CD34;
Vascular endothelial growth factors;
Endothelin-1
- From:
Chinese Journal of General Surgery
2019;34(11):932-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the roles of CD34, VEGF and endothelin-1 in hepatic sinusoid capillarization and the intrahepatic metastasis of hepatocellular carcinoma.
Methods:Data of 10 cases of hepatocellular carcinoma without intrahepatic metastasis were compared with those 10 cases of hepatocellular carcinoma with intrahepatic metastasis . Liver cancer tissues and paracancerous tissues were collected, hepatic sinusoidal capillarization was detected by immunohistochemistry, mRNA expression and protein expression of CD34, VEGF and endothelin-1 genes were detected by QPCR, Western blot, and the relationship between gene expression and metastasis of HCC was observed.
Results:Hepatic sinusoid capillarization was observed in carcinoma tissues, not in the adjacent tissues, whereas mRNAs and proteins of CD34, VEGF and endothelin-1 were higher in liver cancer tissues than that in the adjacent tissuess. In addition, both mRNA and protein expression levels of these genes were significantly higher in the intrahepatic metastatic liver cancers than those without metastasis (P<0.05).
Conclusion:CD34, VEGF and endothelin - 1 may play important roles in intrahepatic metastasis of liver cancer.
