The application of multi-point puncture D2-40, CD31, CD34 staining for autologous skin grafting in Kaposiform hemangioendothelioma
10.3760/cma.j.issn.1009-4598.2019.11.009
- VernacularTitle: 多点穿刺D2-40、CD31、CD34免疫组织化学染色在卡波西样血管内皮瘤原位植皮术中的应用
- Author:
Longlong SUN
1
;
Bin SUN
1
;
Yaping XING
2
;
Yuchun MA
1
;
Hongzhao LEI
1
;
Miaomiao LI
1
;
Changxian DONG
1
Author Information
1. Department of Hemangioma Surgery in People′s Hospital of Zhengzhou University and Henan Provincial People′s Hospital, Zhengzhou 450000, China
2. Department of Pathology in Henan Provincial People′s Hospital, Zhengzhou 450000, China
- Publication Type:Clinical Trail
- Keywords:
Kaposiform hemangioendothelioma;
Autologous skin grafting;
Immunohisto-chemistry
- From:
Chinese Journal of Plastic Surgery
2019;35(11):1102-1106
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the feasibility of application of multi-point puncture immunohistochemical to determine the invasion extent of the epidermis in Kaposiform hemangioendothelioma before surgery and choose the surgical method.
Methods:From July 2013 to April 2016, 17 patients with Kaposiform hemangioendothelioma were admitted to the People′s Hospital of Zhengzhou University, including 7 males and 10 females. The first consultation age was 75.60±31.55 days. Preoperative multi-point pathological puncture D2-40, CD31, CD34 immunohistochemical staining was to test the invasion extent of the epidermis in Kaposiform hemangioendothelioma and to determine whether undergo autologous skin grafting. Skin graft survival areas were recorded postoperatively. Platelet values were examined after 1 week, 4 weeks, 12 weeks and 24 weeks.
Results:In these 17 cases, 15 cases with epidermis and dermis unaffected was performed in situ grafting. 2 cases with the tumor invasion, superficial skin grafting were repaired. In these 15 cases of in situ grafting, platelet values returned to normal at 1 week after the operations, and platelet values were higher than 100×109/L at 4 weeks, 12 weeks and 24 weeks. Among them, 12 cases of epidermal survival area were greater than 90%, 2 cases were 70%-90%, and 1 cases was about 50%.
Conclusions:During the complete resection of Kaposiform hemangioendothelioma, the multi-puncture D2-40, CD31 and CD34 staining are used to determine invasion extent of the tumor to the skin, which can maximize the retention of normal tissue surface, reduce postoperative complications. This is a new multi-disciplinary surgical approach.
