Nano-emulsion as a vaccine adjuvant can enhance the humoral immunity against influenza in aged and young mice
10.3760/cma.j.issn.0254-5101.2019.11.010
- VernacularTitle: 纳米乳剂作为疫苗佐剂可增强老龄鼠和年轻鼠的抗流感体液免疫
- Author:
Pu SHAN
1
;
Zhibiao WANG
1
;
Duoqian WEI
1
;
Shaojie HAO
1
;
Dexiang CHEN
2
;
Jing XU
1
Author Information
1. The Third Laboratory, National Vaccine & Serum Institute, Beijing 101111, China
2. Chengdu Maikekang BioTechnology Co., Ltd., Chengdu 610041, China
- Publication Type:Journal Article
- Keywords:
Adjuvant;
Influenza;
Nano-emulsion adjuvant;
Aged mice;
Immunogenicity
- From:
Chinese Journal of Microbiology and Immunology
2019;39(11):869-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the immunogenicity of split influenza H1N1 vaccine formulated with an oil-in-water nano-emulsion adjuvant in aged mice and young mice.
Methods:A nano-emulsion adjuvant formulated split influenza H1N1 vaccine was used to immunize aged and young mice through intramuscular injection. Each mouse was immunized with 0.012 μg of hemagglutinin (HA) twice with an interval of 28 d. Hemagglutination inhibition (HI) titers in serum were measured 27 d after first immunization. Serum HI, IgG1 and IgG2a titers were detected 14 d after the last immunization. No adjuvant-formulated vaccine and normal saline (NS) were used to set up control groups. Virus challenge test was carried out using 10 times the median lethal dose (LD50) of A/Puerto Rico/8/34 (H1N1) strain two weeks after the last immunization and the protective effects were assessed through measuring the dynamic changes in body weight and survival rate.
Results:Higher levels of serum HI, IgG1 and IgG2a antibodies and higher HI antibody conversion rates were induced in the adjuvant groups, especially in the aged mice group, than in the control groups. Nano-emulsion adjuvant improved the immunogenicity of HA and mouse immunity to A/Puerto Rico/8/34 (H1N1).
Conclusions:Nano-emulsion adjuvant could enhance the immunogenicity of influenza antigens, especially in aged mice.
