Role of lncRNA-mtb in Mycobacterium tuberculosis-infected macrophages
10.3760/cma.j.issn.0254-5101.2019.11.007
- VernacularTitle: lncRNA-mtb在结核分枝杆菌感染巨噬细胞中的功能研究
- Author:
Zichao JIA
1
;
Zhidong HU
1
;
Wei LUO
1
,
2
;
Qian WANG
3
Author Information
1. Department of Clinical Laboratory, General Hospital of Tianjin Medical University, Tianjin 300052, China
2. School of Laboratory, Tianjin Medical University, Tianjin 300050, China
3. Key Laboratory of TCM for Infectious Diseases Prevention and Control of State Administration of Traditional Chinese Medicine of the People′s Republic of China, Department of Pathology, Tianjin Haihe Hospital, Tianjin 300050, China
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
lncRNA-mtb;
Macrophage;
Inflammation
- From:
Chinese Journal of Microbiology and Immunology
2019;39(11):848-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of lncRNA-mtb in Mycobacterium tuberculosis (M.tuberculosis)-infected macrophages.
Methods:A predicting software and Western blot assay were used to verify the non-coding nature of lncRNA-mtb. RT-qPCR was performed to detect lncRNA-mtb expression in macrophages infected by different Mycobacteria. Cytokine secretion was detected with ELISA after silencing the expression of lncRNA-mtb in macrophages with RNA interference (RNAi) technology. Western blot and RT-qPCR were performed to analyze whether the activation of macrophage inflammation could be induced by lncRNA-mtb during M. tuberculosis infection. Effects of lncRNA-mtb silencing on the bactericidal activity of M. tuberculosis-infected macrophages were evaluated.
Results:Increased expression of lncRNA-mtb was observed in macrophages infected with M. tuberculosis H37Rv or H37Ra. After silencing the expression of lncRNA-mtb, IL-1β secretion in H37Rv infected-macrophages was significantly decreased. Further studies revealed that lncRNA-mtb might be involved in the activation of NLRP3 in M. tuberculosis-infected macrophages. The bactericidal activity of macrophages against H37Ra was impaired after silencing the expression of lncRNA-mtb.
Conclusions:Enhanced expression of lncRNA-mtb could be induced in macrophages infected with H37Rv or H37Ra. Moreover, lncRNA-mtb was involved in M. tuberculosis-induced inflammation in cells. Silencing lncRNA-mtb wolud attenuate the ability of macrophages to clear M. tuberculosis.
