Sequence analysis and 3D molecular structure simulation of a novel HLA allele B*51: 159
10.3760/cma.j.issn.1003-9406.2019.11.020
- VernacularTitle: HLA新等位基因B*51：159的序列分析及三维分子结构模拟
- Author:
Wenben QIAO
1
;
Yonghong SONG
;
Xiangmin NIE
;
Yan LIU
;
Haifeng ZHU
;
Yi ZHANG
Author Information
1. Blood Center of Shandong Province, Jinan, Shandong 250014, China
- Publication Type:Clinical Trail
- Keywords:
Human leukocyte antigen;
Novel allele;
B*51: 159;
Three dimensional structure
- From:
Chinese Journal of Medical Genetics
2019;36(11):1133-1135
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify a novel human leukocyte antigen (HLA) B allele in a Chinese Han individual and construct its three-dimensional structure.
Methods:The initial HLA genotyping was performed by PCR-sequence-based typing (PCR-SBT). The ambiguous allele was confirmed with single-strand DNA sequencing. The DNA sequence was analyzed to identify the difference between the novel allele and its closest matching allele. Finally, the three-dimensional molecular structure of the novel allele was constructed using a Swiss-Model.
Results:One allele of the subject at the HLA-B locus was B*44: 03: 01, whilst the other was a novel allele which differed from the closest matching allele B*51: 01: 01: 01 by nucleotide (nt) 329 A>C in exon 2, resulting in an amino acid change at codon 86 (p.Asn86Thr).
Conclusion:A novel HLA-B allele has been identified and officially named as HLA-B*51: 159 by the WHO Nomenclature Committee for Factors of the HLA System. The three-dimensional structure of B*51: 159 was simulated.
