Application of multiple MLL gene rearrangement detection techniques for children with acute mononuclear leukemia
10.3760/cma.j.issn.1003-9406.2019.11.005
- VernacularTitle: 多种MLL基因重排检测技术在儿童急性单核系白血病中的应用
- Author:
Ding ZHAO
1
;
Rui LI
;
Yaodong ZHANG
;
Jijun SONG
;
Zhenhua ZHANG
;
Meiye WANG
;
Chaojie WANG
;
Jin WANG
Author Information
1. Henan Provincial Key Laboratory for Genetics and Metabolic Diseases in Childhood, Children’s Hospital Affiliated of Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospital, Zhengzhou, Henan 450018, China
- Publication Type:Journal Article
- Keywords:
MLL gene;
Acute mononuclear leukemia;
Fluorescence in situ hybridization;
Reverse transcription PCR
- From:
Chinese Journal of Medical Genetics
2019;36(11):1077-1080
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the value of detecting multiple rearrangements of MLL gene in children with acute mononuclear leukemia (AML).
Methods:Eighty six children with AML were analyzed by fluorescence in situ hybridization (FISH), chromosomal karyotyping and multiplex reverse transcription-PCR (RT-PCR).
Results:Cross signals were detected by FISH in 26 cases, and 30.2% were detected with MLL gene rearrangements. R-band karyotyping analysis revealed 14 translocations with breakages involving 11q23 and 5 other aberrations, which yielded an overall detection rate of 22.1%. Multiple RT-PCR has detected 12 fusion genes produced by the MLL translocation, which yielded a detection rate of 14.0%. A significant difference was found in the detection rate of the three methods (P<0.05).
Conclusion:Combined use of FISH, chromosomal karyotyping and multiplex RT-PCR can improve the detection of MLL gene rearrangements and provide important clues for clinical diagnosis, treatment and prognosis of AML.
