The expression of LINC00052 during glycidyl methacrylate-induced malignant transformation of 16HBE cells
10.3760/cma.j.issn.1001-9391.2019.11.002
- VernacularTitle: LINC00052在甲基丙烯酸环氧丙酯诱导人支气管上皮细胞恶性转化过程中的表达
- Author:
Quankai WANG
1
,
2
;
Haoran GUO
3
;
Guangyun XIE
1
,
2
;
Shunpeng MA
1
,
2
;
Baolier WUHAN
1
;
Jiayang SONG
1
;
Jianning XU
1
,
2
Author Information
1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China
2. Key Laboratory of Chemical Safety and Health Chinese Center for Disease Control and Prevention, Beijing 100050, China
3. Jiangyin Municipal Center for Disease Control and Prevention, Jiangyin 214431, China
- Publication Type:Journal Article
- Keywords:
Epithelial cells;
Glycidyl methacrylate;
LncRNA;
LINC00052;
Malignant transformation
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(11):806-809
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and role of LINC00052 during glycidyl methacrylate (GMA) -induced malignant transformation of 16HBE cells.
Methods:Human bronchial epithelial (16HBE) cells were divided into GMA transformation group and corresponding DMSO control group, and the 10th, 20th and 30th generation cells of each group were collected LncRNA microarrays were used to analysis expression of LINC00052 in different stage of malignant transformation. Bioinformatics analysis was applied and the relative expression of LINC00052 and its potentially target genes was detected by real-time quantification PCR (qPCR) .
Results:The results of microarray analysis showed that LINC00052 was up-regulated by 1.32-fold, down-regulated by 1.64-fold and down-regulated by 4.92-fold in the malignant transformation early (P10) , middle term (P20) and late (P30) , respectively, The results of qPCR showed that compared with the DMSO control group, the expression of LINC00052 was up-regulated by 1.55 times, down-regulated by 1.20 times and down-regulated by 2.35 times in P10, P20 and P30, respectively, and the difference was statistically significant (P<0.05) . There was a statistically significant difference in the relative expression of NTRK3 between the GMA transformation group of P10 and P30 generations with the corresponding DMSO control group (P<0.05) .
Conclusion:LINC00052 is highly expressed in early time of GMA-induced malignant transformation of 16HBE, and down-regulated in the middle and last stage of malignant transformation and may play a protective role in GMA-induced malignant transformation of 16HBE by influencing the expression of its target gene NTRK3.
