Molecular Strain Typing of Mycobacterium tuberculosis: a Review of Frequently Used Methods.
10.3346/jkms.2016.31.11.1673
- Author:
Phyu Win EI
1
;
Wah Wah AUNG
;
Jong Seok LEE
;
Go Eun CHOI
;
Chulhun L CHANG
Author Information
1. Advanced Molecular Research Centre, Department of Medical Research, Yangon, Myanmar.
- Publication Type:Review
- Keywords:
Tuberculosis;
Mycobacterium tuberculosis;
Molecular Strain Typing
- MeSH:
Base Sequence;
Dermatoglyphics;
Genome;
Global Health;
Methods*;
Molecular Typing;
Mycobacterium tuberculosis*;
Mycobacterium*;
Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Repetitive Sequences, Nucleic Acid;
Tandem Repeat Sequences;
Tuberculosis
- From:Journal of Korean Medical Science
2016;31(11):1673-1683
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, remains one of the most serious global health problems. Molecular typing of M. tuberculosis has been used for various epidemiologic purposes as well as for clinical management. Currently, many techniques are available to type M. tuberculosis. Choosing the most appropriate technique in accordance with the existing laboratory conditions and the specific features of the geographic region is important. Insertion sequence IS6110-based restriction fragment length polymorphism (RFLP) analysis is considered the gold standard for the molecular epidemiologic investigations of tuberculosis. However, other polymerase chain reaction-based methods such as spacer oligonucleotide typing (spoligotyping), which detects 43 spacer sequence-interspersing direct repeats (DRs) in the genomic DR region; mycobacterial interspersed repetitive units–variable number tandem repeats, (MIRU-VNTR), which determines the number and size of tandem repetitive DNA sequences; repetitive-sequence-based PCR (rep-PCR), which provides high-throughput genotypic fingerprinting of multiple Mycobacterium species; and the recently developed genome-based whole genome sequencing methods demonstrate similar discriminatory power and greater convenience. This review focuses on techniques frequently used for the molecular typing of M. tuberculosis and discusses their general aspects and applications.
