Proteomics analysis of hippocampus and striatum in rats with hyperglycemia using iTRAQ technique
10.3760/cma.j.issn.1000-6699.2019.11.010
- VernacularTitle: 基于iTRAQ技术的糖尿病SD大鼠海马纹状体区差异蛋白组学研究
- Author:
Ablat DILMURAT
1
;
Xinling YANG
2
;
Yumin JIA
3
;
Feifei GENG
3
Author Information
1. First Aid Centre, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
2. Department of Neurology, The Second Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China
3. Fourth Branch of Comprehensive of Department of Internal Medicine, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China
- Collective Name:Fourth Branch of Comprehensive of Department of Internal Medicine, The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Proteomics analysis;
Isobaric tags for relative and absolute quantification;
2-dimensional liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(11):973-980
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen the deferentially expressed proteins in hippocampus and striatum in rat models of diabetes mellitus and normal SD rats, and to elucidate the effects of hyperglycemia on central nervous system.
Methods:SD rats were randomly divided into normal group(n=10)and hyperglycemia group(n=15); rat models of diabetes were induced by the high-fat and high-sugar diet combined with single intraperitoneal injection of streptozotocin. The hippocampus and striatum tissues from hyperglycemia rat and normal SD rat(control group) were selected as specimens. Isobaric tags for relative and absolute quantification(iTRAQ) technique and 2-dimensional liquid chromatography-tandem mass spectrometry(2D-LC-MS/MS) were used for identifying the deferentially expressed proteins. The deferentially expressed proteins were analyzed by bio-informatics for searching candidate proteins.Finally, the candidate proteins were verified by Western blotting.
Results:A total of 347 proteins showed significantly different expressions, which included 139 up-regulated proteins and 289 down-regulated proteins. These proteins included metabolic glutamate receptor 2, ferritin, leucine repeat protein and complex 2, which are related to Parkinson′s disease. KEEN search indicated that the pathway of dopaminergic synaptic, complement, gap junction, autophagic and Fcgamma R-mediated phagocytosis were involved with these significant differentially expressed proteins. The protein interaction network showed that ferrin, aldehyde/ketone reductase(ARK), Ca2+ -binding protein(CaBP), and protein tyrosine phosphatase(PTP) were located at the crossed nodes. The expression levels of ferrin, ARK, PTP, and CaBP, which were verified by Western blotting, and the results were consistent with those of the quantitative mass spectrometry(P<0.05).
Conclusion:Deferentially expressed proteins in hippocampus striatum of diabetic rats can be effectively screened by iTRAQ technique combined with 2D-LC-MS/MS. It may provide new clues for the mechanism of neurodegenerative disease.
